Home Primary Antibodies Phospho-mTOR (Ser2481) Antibody [H20P20]

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Phospho-mTOR (Ser2481) Antibody [H20P20]

Cat.No.: F1578

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000 - 1:10000
    Application
    WB
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    289 kDa 289 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.
    Positive Control HeLa cells (serum-starved, overnight; PMA, 200 nM, 4 h); HEK-293 cells
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-mTOR (Ser2481) Antibody [H20P20] detects endogenous levels of total mTOR protein only when it is phosphorylated at Ser2481.
    Clone
    H20P20
    Synonym(s)
    mTOR; Serine/threonine-protein kinase mTOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; FRAP1; FRAP2; RAFT1; RAPT1
    Background
    Phospho-mTOR (Ser2481) represents the autophosphorylated form of the central Ser/Thr kinase mTOR, serving as a specific, vertebrate-specific biomarker for the assembly and catalytic activity of the rapamycin-insensitive mTORC2 complex. Located at the C-terminal regulatory tail between the kinase and FATC domains, Ser2481 phosphorylation requires the intact mTORC2 components Rictor and mSin1 and acts as a direct monitor of mTORC2-specific activity, generally unaffected by acute, short-term rapamycin treatment but disrupted by prolonged exposure that inhibits complex assembly. While mTORC1 is predominantly marked by PI3K/Akt-dependent phosphorylation at Ser2448, phosphorylation at Ser2481 in mTORC2 is crucial for the regulation of AGC kinases, enabling the phosphorylation of Akt at Ser473, PKCα for cytoskeleton organization, and SGK1 for survival. Consequently, this modification supports insulin/PI3K signaling, metabolism, and anabolism independently of mTORC1's translational control, promoting cell proliferation and survival. In breast and renal carcinomas, increased phospho-Ser2481 serves as a more reliable indicator of active mTORC2 than Akt Ser473 itself, directly predicting sensitivity to rapalogs and the necessity of utilizing dual mTORC1/2 inhibitors for effective therapy.
    References
    • https://pubmed.ncbi.nlm.nih.gov/20022946/
    • https://pubmed.ncbi.nlm.nih.gov/19244117/

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