Phospho-MOB1 (Thr12) Antibody [F15P10]

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Ramos, Lane 2: Ramos (λ phosphatase treated)

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
24 kDa

Datasheet & SDS

Biological Description

Specificity
Phospho-MOB1 (Thr12) Antibody [F15P10] detects endogenous levels of total MOB1 protein only when it is phosphorylated at Thr12.

Clone
F15P10

Synonym(s)
MOB kinase activator 1A; Mob1α; MOBKL1A; MOB1A

Background
Phospho-MOB1 (Thr12) designates the activated conformation of MOB1A/B adaptor proteins central to the Hippo tumor suppressor pathway that governs organ size, proliferation, and apoptosis through kinase cascade amplification. MOB1 organizes a compact globular fold with an N-terminal extension and core domain that undergoes phosphorylation at Thr12 by MST1/2 kinases, stabilizing an open conformation where the Switch helix dissociates to expose binding interfaces for LATS1/2 N-terminal regulatory regions. This phospho-Thr12 event allosterically activates LATS1/2 catalytic domains through dynamic scaffolding that juxtaposes MST-phosphorylated activation loops with MOB1 phospho-sites, enabling trans-phosphorylation that ramps up YAP/TAZ inhibitory serine phosphorylation for cytoplasmic retention and proteasomal degradation via β-TRCP ubiquitin ligase. Phospho-MOB1 further engages NDR1/2 kinases with differential affinity modulated by Thr12/Thr35 phosphates that pull N-terminal strands into β-sheet configurations stabilizing kinase-MOB complexes for mitotic exit and ploidy control. The resultant LATS-YAP/TAZ axis integrates upstream inputs, such as cell density and mechanotransduction, through Merlin-expanded sensing to transcriptionally repress CTGF, Cyr61, and c-Myc, thereby facilitating contact inhibition during epithelial morphogenesis. Phospho-MOB1 coordinates tissue homeostasis in liver regeneration and neural tube closure, with ubiquitous expression ensuring robust pathway fidelity across proliferative niches.

Background

Phospho-MOB1 (Thr12) designates the activated conformation of MOB1A/B adaptor proteins central to the Hippo tumor suppressor pathway that governs organ size, proliferation, and apoptosis through kinase cascade amplification. MOB1 organizes a compact globular fold with an N-terminal extension and core domain that undergoes phosphorylation at Thr12 by MST1/2 kinases, stabilizing an open conformation where the Switch helix dissociates to expose binding interfaces for LATS1/2 N-terminal regulatory regions. This phospho-Thr12 event allosterically activates LATS1/2 catalytic domains through dynamic scaffolding that juxtaposes MST-phosphorylated activation loops with MOB1 phospho-sites, enabling trans-phosphorylation that ramps up YAP/TAZ inhibitory serine phosphorylation for cytoplasmic retention and proteasomal degradation via β-TRCP ubiquitin ligase. Phospho-MOB1 further engages NDR1/2 kinases with differential affinity modulated by Thr12/Thr35 phosphates that pull N-terminal strands into β-sheet configurations stabilizing kinase-MOB complexes for mitotic exit and ploidy control. The resultant LATS-YAP/TAZ axis integrates upstream inputs, such as cell density and mechanotransduction, through Merlin-expanded sensing to transcriptionally repress CTGF, Cyr61, and c-Myc, thereby facilitating contact inhibition during epithelial morphogenesis. Phospho-MOB1 coordinates tissue homeostasis in liver regeneration and neural tube closure, with ubiquitous expression ensuring robust pathway fidelity across proliferative niches.

References
https://pubmed.ncbi.nlm.nih.gov/27335147/ https://pubmed.ncbi.nlm.nih.gov/26108669/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%