Phospho-c-Kit (Tyr703) Antibody [K16C2]

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
145 kDa

Positive Control	H526 cells (SCF-treated)
Negative Control	H526 cells

Datasheet & SDS

Biological Description

Specificity
Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb detects endogenous levels of total c-Kit protein only when it is phosphorylated at Tyr703.

Clone
K16C2

Synonym(s)
Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117

Background
Phospho-c-Kit (Tyr703) marks the activated state of c-Kit, a type III receptor tyrosine kinase (RTK) essential for the survival, proliferation, and differentiation of hematopoietic stem cells, mast cells, melanocytes, and germ cells. c-Kit comprises five immunoglobulin-like extracellular domains for stem cell factor (SCF) binding, a transmembrane domain, a juxtamembrane (JM) domain, and a split kinase domain containing a kinase insert (KI) with Tyr703. Upon SCF engagement, the receptor dimerizes and undergoes sequential autophosphorylation, starting at JM residues (Tyr568/570) to relieve auto-inhibition, followed by phosphorylation of KI sites, including Tyr703. Specifically, phosphorylation at Tyr703 creates a crucial docking site for the adaptor protein Grb2, which initiates the Ras-ERK MAPK pathway to promote cellular proliferation and survival. While normal signaling regulates essential processes like hematopoiesis and melanogenesis, dysregulation through gain-of-function mutations or overexpression leads to constitutive Tyr703 phosphorylation, driving oncogenesis in gastrointestinal stromal tumors (GIST), mastocytosis, acute myeloid leukemia (AML), and melanoma.

Background

Phospho-c-Kit (Tyr703) marks the activated state of c-Kit, a type III receptor tyrosine kinase (RTK) essential for the survival, proliferation, and differentiation of hematopoietic stem cells, mast cells, melanocytes, and germ cells. c-Kit comprises five immunoglobulin-like extracellular domains for stem cell factor (SCF) binding, a transmembrane domain, a juxtamembrane (JM) domain, and a split kinase domain containing a kinase insert (KI) with Tyr703. Upon SCF engagement, the receptor dimerizes and undergoes sequential autophosphorylation, starting at JM residues (Tyr568/570) to relieve auto-inhibition, followed by phosphorylation of KI sites, including Tyr703. Specifically, phosphorylation at Tyr703 creates a crucial docking site for the adaptor protein Grb2, which initiates the Ras-ERK MAPK pathway to promote cellular proliferation and survival. While normal signaling regulates essential processes like hematopoiesis and melanogenesis, dysregulation through gain-of-function mutations or overexpression leads to constitutive Tyr703 phosphorylation, driving oncogenesis in gastrointestinal stromal tumors (GIST), mastocytosis, acute myeloid leukemia (AML), and melanoma.

References
https://pubmed.ncbi.nlm.nih.gov/10377264/ https://pubmed.ncbi.nlm.nih.gov/22437942/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%