PGK1 Antibody [H17D18] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: C6, Lane 4: HuH7

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
43 kDa

Datasheet & SDS

Biological Description

Specificity
PGK1 Antibody [H17D18] detects endogenous levels of total PGK1 protein.

Clone
H17D18

Synonym(s)
Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2 (PRP 2); PGK1; PGKA

Background
PGK1 (phosphoglycerate kinase 1) is a core glycolytic enzyme that catalyzes the reversible transfer of a phosphate group from 1,3‑bisphosphoglycerate to ADP, generating ATP and 3‑phosphoglycerate and thereby linking the upper and lower halves of the glycolytic pathway in most mammalian cells. PGK1 also functions as a non‑canonical protein kinase that is itself regulated by post‑translational modifications, including autophosphorylation at Tyr324, which is required for its full activation and is reversed by the lipid and protein phosphatase PTEN; loss of PTEN in tumor cells consequently elevates phospho‑Tyr324‑PGK1, enhances glycolytic flux, and promotes tumor growth. In hypoxic or nutrient‑stressed conditions, PGK1 can translocate to the mitochondria where it phosphorylates the pyruvate dehydrogenase kinase PDK1 at Thr338, inhibiting the pyruvate dehydrogenase complex, suppressing mitochondrial pyruvate oxidation, and favoring lactate production and glycolytic dependency. Under glutamine‑starved or hypoxic stress, acetylated PGK1 interacts with Beclin1 and phosphorylates it to activate the VPS34 complex and initiate autophagy, thereby coordinating metabolic adaptation with selective degradation and recycling of cytoplasmic components. PGK1 expression and activity are upregulated in multiple cancer types, where its dual glycolytic and kinase functions support proliferation, invasion, and stress survival, and altered PTEN‑dependent regulation of PGK1 Tyr324‑phosphorylation is linked to aggressive brain‑tumor phenotypes and poor clinical outcomes.

Background

PGK1 (phosphoglycerate kinase 1) is a core glycolytic enzyme that catalyzes the reversible transfer of a phosphate group from 1,3‑bisphosphoglycerate to ADP, generating ATP and 3‑phosphoglycerate and thereby linking the upper and lower halves of the glycolytic pathway in most mammalian cells. PGK1 also functions as a non‑canonical protein kinase that is itself regulated by post‑translational modifications, including autophosphorylation at Tyr324, which is required for its full activation and is reversed by the lipid and protein phosphatase PTEN; loss of PTEN in tumor cells consequently elevates phospho‑Tyr324‑PGK1, enhances glycolytic flux, and promotes tumor growth. In hypoxic or nutrient‑stressed conditions, PGK1 can translocate to the mitochondria where it phosphorylates the pyruvate dehydrogenase kinase PDK1 at Thr338, inhibiting the pyruvate dehydrogenase complex, suppressing mitochondrial pyruvate oxidation, and favoring lactate production and glycolytic dependency. Under glutamine‑starved or hypoxic stress, acetylated PGK1 interacts with Beclin1 and phosphorylates it to activate the VPS34 complex and initiate autophagy, thereby coordinating metabolic adaptation with selective degradation and recycling of cytoplasmic components. PGK1 expression and activity are upregulated in multiple cancer types, where its dual glycolytic and kinase functions support proliferation, invasion, and stress survival, and altered PTEN‑dependent regulation of PGK1 Tyr324‑phosphorylation is linked to aggressive brain‑tumor phenotypes and poor clinical outcomes.

References
https://pubmed.ncbi.nlm.nih.gov/28486006/ https://pubmed.ncbi.nlm.nih.gov/31492635/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%