Pax6 Antibody [H2K10] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Lane 1: 293T, Lane 2: SW480, Lane 3: RAW264.7, Lane 4: INS-1

Usage Information

Dilution
WB1:1000 IP1:200 IHC1:250-1:1000 IF1:100-1:400

Application
WB, IP, IHC, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50 kDa

Datasheet & SDS

Biological Description

Specificity
Pax6 Antibody [H2K10] detects endogenous levels of total Pax6 protein.

Clone
H2K10

Synonym(s)
Paired box protein Pax-6; Oculorhombin; PAX6

Background
Pax6 belongs to the subgroup III paired box transcription factor family characterized by paired and homeodomains that orchestrate tissue-specific gene expression during embryogenesis and lineage maintenance. The protein features an N-terminal paired domain with two helix-turn-helix subdomains for high-affinity binding to consensus TAAT/TCAT motifs alongside a C-terminal homeodomain that cooperates through cooperative DNA contacts and transactivation domains rich in acidic residues. Pax6 maintains radial glial progenitor pools by repressing cell cycle exit genes through direct promoter occupancy at Cdkn1a and Cdkn1b while promoting Wnt3a expression to modulate radial migration via non-canonical planar cell polarity signaling that aligns radial glia scaffolds with cortical layering. Temporal gradients of Pax6 dosage fine-tune G1 phase lengthening through oscillating Cdk6 levels, balancing proliferation with differentiation timing as progenitors transition from symmetric to asymmetric divisions during upper layer neuron generation. Activation occurs via upstream Six3 and Fgf signaling cascades that prime paired domain accessibility, with autoregulatory loops sustaining high rostral expression through chromatin looping to its own enhancer. In pancreatic beta cells driven by Pdx1 and Ngn3 co-expression, Pax6 binds islet-specific enhancers to transactivate insulin biosynthesis via coordination with Pdx1 at the Ins1/2 promoters while regulating glucose-stimulated ATP production through Slc2a2 and Abcc8 control, ensuring Ca2+ dynamics and GLP-1 responsiveness for mature secretory competence.

Background

Pax6 belongs to the subgroup III paired box transcription factor family characterized by paired and homeodomains that orchestrate tissue-specific gene expression during embryogenesis and lineage maintenance. The protein features an N-terminal paired domain with two helix-turn-helix subdomains for high-affinity binding to consensus TAAT/TCAT motifs alongside a C-terminal homeodomain that cooperates through cooperative DNA contacts and transactivation domains rich in acidic residues. Pax6 maintains radial glial progenitor pools by repressing cell cycle exit genes through direct promoter occupancy at Cdkn1a and Cdkn1b while promoting Wnt3a expression to modulate radial migration via non-canonical planar cell polarity signaling that aligns radial glia scaffolds with cortical layering. Temporal gradients of Pax6 dosage fine-tune G1 phase lengthening through oscillating Cdk6 levels, balancing proliferation with differentiation timing as progenitors transition from symmetric to asymmetric divisions during upper layer neuron generation. Activation occurs via upstream Six3 and Fgf signaling cascades that prime paired domain accessibility, with autoregulatory loops sustaining high rostral expression through chromatin looping to its own enhancer. In pancreatic beta cells driven by Pdx1 and Ngn3 co-expression, Pax6 binds islet-specific enhancers to transactivate insulin biosynthesis via coordination with Pdx1 at the Ins1/2 promoters while regulating glucose-stimulated ATP production through Slc2a2 and Abcc8 control, ensuring Ca2+ dynamics and GLP-1 responsiveness for mature secretory competence.

References
https://pubmed.ncbi.nlm.nih.gov/25805971/ https://pubmed.ncbi.nlm.nih.gov/28377501/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%