PAF1 Antibody [D19B13] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Hela, Lane 2: PANC-1, Lane 3: RAW264.7, Lane 4: H-4-II-E

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
80 kDa

Datasheet & SDS

Biological Description

Specificity
PAF1 Antibody [D19B13] detects endogenous levels of total PAF1 protein.

Clone
D19B13

Synonym(s)
RNA polymerase II-associated factor 1 homolog; hPAF1; Pancreatic differentiation protein 2; PAF1; PD2

Background
Paf1 (RNA polymerase II–associated factor 1) is a core subunit of the conserved Paf1 complex (Paf1C), a multifunctional elongation‑associated module that physically associates with the elongating RNA polymerase II (RNAPII) machinery and functions as a platform coordinating transcription, chromatin modification, and RNA‑processing events. The Paf1 complex contains Paf1, Ctr9, Cdc73, Leo1, Rtf1, and, in higher eukaryotes, Ski8, and it travels with RNAPII across the body of actively transcribed genes, where it interacts with Spt4–Spt5, FACT, and other elongation factors to influence polymerase processivity and chromatin integrity. A key mechanistic function of the Paf1C is stimulation of histone H2B ubiquitination, which in turn promotes histone H3 methylation and modulates nucleosome dynamics around transcribed regions, thereby shaping the chromatin landscape that RNAPII must traverse. Paf1C further couples transcription elongation to mRNA 3′‑end processing by recruiting the cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) complexes via the Cdc73 subunit, ensuring co‑transcriptional assembly of the 3′‑end‑processing machinery at gene termini. In addition, the Ski8 subunit of the Paf1C links the complex to the TRAMP/Ski/Exosome RNA‑surveillance network, enabling co‑transcriptional quality control and degradation of aberrant or non‑productive transcripts.

Background

Paf1 (RNA polymerase II–associated factor 1) is a core subunit of the conserved Paf1 complex (Paf1C), a multifunctional elongation‑associated module that physically associates with the elongating RNA polymerase II (RNAPII) machinery and functions as a platform coordinating transcription, chromatin modification, and RNA‑processing events. The Paf1 complex contains Paf1, Ctr9, Cdc73, Leo1, Rtf1, and, in higher eukaryotes, Ski8, and it travels with RNAPII across the body of actively transcribed genes, where it interacts with Spt4–Spt5, FACT, and other elongation factors to influence polymerase processivity and chromatin integrity. A key mechanistic function of the Paf1C is stimulation of histone H2B ubiquitination, which in turn promotes histone H3 methylation and modulates nucleosome dynamics around transcribed regions, thereby shaping the chromatin landscape that RNAPII must traverse. Paf1C further couples transcription elongation to mRNA 3′‑end processing by recruiting the cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) complexes via the Cdc73 subunit, ensuring co‑transcriptional assembly of the 3′‑end‑processing machinery at gene termini. In addition, the Ski8 subunit of the Paf1C links the complex to the TRAMP/Ski/Exosome RNA‑surveillance network, enabling co‑transcriptional quality control and degradation of aberrant or non‑productive transcripts.

References
https://pubmed.ncbi.nlm.nih.gov/19164765/ https://pubmed.ncbi.nlm.nih.gov/22408743/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%