NUP153 Antibody [M17C21] | Primary Antibodies

Application: Reactivity: Human, Monkey

Lane 1: K562, Lane 2: MCF7, Lane 3: A549, Lane 4: COS-7

Usage Information

Dilution
WB1:1000 IF1:400

Application
WB, IF

Reactivity
Human, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
180 kDa

Datasheet & SDS

Biological Description

Specificity
NUP153 Antibody [M17C21] detects endogenous levels of total NUP153 protein.

Clone
M17C21

Synonym(s)
Nucleoporin Nup153; 153 kDa nucleoporin; NUP153

Background
NUP153 is a nuclear basket–associated nucleoporin that forms part of the nuclear pore complex (NPC), a multi‑protein channel embedded in the nuclear envelope that mediates selective nucleocytoplasmic transport of proteins, mRNAs, and ribonucleoprotein complexes. NUP153 contains an N‑terminal channel‑interacting domain, a central region that mediates interactions with other nucleoporins, and a C‑terminal FG‑repeat and DNA‑binding‑like module that projects from the nuclear face of the NPC and can contact chromatin and transcription‑regulatory complexes. NUP153 functions as a scaffold for the nuclear basket, helping to organize transport receptors and soluble factors near the NPC and contributing to mRNA quality control by retaining unspliced or improperly processed transcripts within the nucleus. NUP153 binds near transcriptional start sites of developmental and lineage‑specific genes at genomic regions distinct from canonical polycomb‑marked sites, and this binding is required for the recruitment of polycomb repressive complex 1 (PRC1) to a subset of these loci, where PRC1 deposits H2AK119ub1 and maintains transcriptional repression. Depletion of NUP153 in these cells causes derepression of early differentiation‑related genes, precocious activation of neuroectodermal and other lineage programs, and loss of pluripotency without overt disruption of nuclear import of core pluripotency transcription factors, indicating that NUP153 acts as a site‑specific chromatin‑associated regulator rather than solely as a passive transport element.

Background

NUP153 is a nuclear basket–associated nucleoporin that forms part of the nuclear pore complex (NPC), a multi‑protein channel embedded in the nuclear envelope that mediates selective nucleocytoplasmic transport of proteins, mRNAs, and ribonucleoprotein complexes. NUP153 contains an N‑terminal channel‑interacting domain, a central region that mediates interactions with other nucleoporins, and a C‑terminal FG‑repeat and DNA‑binding‑like module that projects from the nuclear face of the NPC and can contact chromatin and transcription‑regulatory complexes. NUP153 functions as a scaffold for the nuclear basket, helping to organize transport receptors and soluble factors near the NPC and contributing to mRNA quality control by retaining unspliced or improperly processed transcripts within the nucleus. NUP153 binds near transcriptional start sites of developmental and lineage‑specific genes at genomic regions distinct from canonical polycomb‑marked sites, and this binding is required for the recruitment of polycomb repressive complex 1 (PRC1) to a subset of these loci, where PRC1 deposits H2AK119ub1 and maintains transcriptional repression. Depletion of NUP153 in these cells causes derepression of early differentiation‑related genes, precocious activation of neuroectodermal and other lineage programs, and loss of pluripotency without overt disruption of nuclear import of core pluripotency transcription factors, indicating that NUP153 acts as a site‑specific chromatin‑associated regulator rather than solely as a passive transport element.

References
https://pubmed.ncbi.nlm.nih.gov/26080816/ https://pubmed.ncbi.nlm.nih.gov/32451376/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%