NTF2 Antibody [A24B24] | Primary Antibodies

Application: Reactivity: Human

Lane 1: Hela, Lane 2: HepG2

Usage Information

Dilution
WB1:500-1:2000 IHC1:250-1:1000

Application
WB, IHC, ELISA

Reactivity
Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
14 kDa 13 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Datasheet & SDS

Biological Description

Specificity
NTF2 Antibody [A24B24] detects endogenous levels of total NTF2 protein.

Clone
A24B24

Synonym(s)
Nuclear transport factor 2; NTF-2; Placental protein 15; PP15; NUTF2; NTF2

Background
NTF2 (nuclear transport factor 2), a highly conserved cytosolic homodimer, serves as the dedicated import receptor for RanGDP, the master regulator of all nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. It features a stable β-barrel structure that forms a deep hydrophobic pocket selectively recognizing the GDP-bound conformation of Ran through critical contacts at switch I/II loops and the C-terminal tail. The core mechanism hinges on NTF2 binding RanGDP with high affinity in the cytoplasm, facilitating its translocation through nuclear pore complexes via interactions with nucleoporins like p62 and Nup50, where RCC1 rapidly exchanges GDP for GTP to establish the essential RanGTP gradient that powers importin-mediated cargo release and exportin loading. This Ran cycle couples directly to cell cycle progression: NTF2-mediated Ran nuclear accumulation prevents G2/M arrest by suppressing the MAD2 spindle assembly checkpoint, ensuring proper kinetochore-microtubule attachments, while post-mitotic NTF2 activity supports NE reassembly by concentrating Ran around chromatin for importin exclusion. NTF2 sustains protein homeostasis across proliferating tissues, making it indispensable for researchers modeling transport defects in temperature-sensitive yeast mutants or quantifying Ran flux via microinjection assays in mammalian cells. Depletion triggers catastrophic nuclear import failure and mitotic catastrophe, with dysregulation implicated in neurodegenerative proteinopathies from aggregate sequestration.

Background

NTF2 (nuclear transport factor 2), a highly conserved cytosolic homodimer, serves as the dedicated import receptor for RanGDP, the master regulator of all nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. It features a stable β-barrel structure that forms a deep hydrophobic pocket selectively recognizing the GDP-bound conformation of Ran through critical contacts at switch I/II loops and the C-terminal tail. The core mechanism hinges on NTF2 binding RanGDP with high affinity in the cytoplasm, facilitating its translocation through nuclear pore complexes via interactions with nucleoporins like p62 and Nup50, where RCC1 rapidly exchanges GDP for GTP to establish the essential RanGTP gradient that powers importin-mediated cargo release and exportin loading. This Ran cycle couples directly to cell cycle progression: NTF2-mediated Ran nuclear accumulation prevents G2/M arrest by suppressing the MAD2 spindle assembly checkpoint, ensuring proper kinetochore-microtubule attachments, while post-mitotic NTF2 activity supports NE reassembly by concentrating Ran around chromatin for importin exclusion. NTF2 sustains protein homeostasis across proliferating tissues, making it indispensable for researchers modeling transport defects in temperature-sensitive yeast mutants or quantifying Ran flux via microinjection assays in mammalian cells. Depletion triggers catastrophic nuclear import failure and mitotic catastrophe, with dysregulation implicated in neurodegenerative proteinopathies from aggregate sequestration.

References
https://pubmed.ncbi.nlm.nih.gov/10930458/ https://pubmed.ncbi.nlm.nih.gov/10679025/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%