research use only

NMDA Receptor 2B/GluN2B Antibody [K20B17]

Cat.No.: F0550

Application: Reactivity: Human, Mouse, Rat

Lane 1: Mouse brain, Lane 2: Neonatal mouse brain

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
190 kDa

Datasheet & SDS

Biological Description

Specificity
NMDA Receptor 2B/GluN2B Antibody [K20B17] detects endogenous levels of total NMDA Receptor 2B/GluN2B protein.

Clone
K20B17

Synonym(s)
Glutamate receptor ionotropic, NMDA 2B; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B (NMDAR2B; NR2B); N-methyl-D-aspartate receptor subunit 3 (NR3; hNR3); GRIN2B; NMDAR2B

Background
NMDA Receptor 2B (GluN2B) is the glutamate-binding subunit critical for assembling functional NMDA receptors, which are heterotetrameric ligand-gated cation channels typically composed of two GluN1 and two GluN2 subunits. Each subunit features an extracellular amino-terminal domain (NTD) and ligand-binding domain (LBD), a membrane-spanning domain (TMD) forming the ion-conducting pore, and an intracellular C-terminal domain (CTD) subject to regulatory phosphorylation. NMDA receptor activation requires concurrent binding of glutamate to GluN2B’s LBD and glycine or D-serine to GluN1, along with membrane depolarization to relieve the Mg²⁺ block. This ligand binding induces LBD clamshell closure, transmitting conformational changes through the TMD to open the channel and permit Na⁺, K⁺, and especially Ca²⁺ influx. GluN2B-containing receptors are especially prominent during early neurodevelopment and at extrasynaptic sites, where they enable synaptic plasticity processes such as long-term potentiation (LTP). Phosphorylation of GluN2B at Tyr1472 by Src family kinases enhances Ca²⁺ permeability and couples receptor activation to CaMKII-mediated spine remodeling and memory formation. While GluN2B-driven signaling is essential for neurodevelopment and learning, its overactivation is implicated in excitotoxic neurodegeneration, such as in Alzheimer’s, Parkinson’s, stroke, and chronic pain, where excessive Ca²⁺ entry triggers cell death pathways.

Background

NMDA Receptor 2B (GluN2B) is the glutamate-binding subunit critical for assembling functional NMDA receptors, which are heterotetrameric ligand-gated cation channels typically composed of two GluN1 and two GluN2 subunits. Each subunit features an extracellular amino-terminal domain (NTD) and ligand-binding domain (LBD), a membrane-spanning domain (TMD) forming the ion-conducting pore, and an intracellular C-terminal domain (CTD) subject to regulatory phosphorylation. NMDA receptor activation requires concurrent binding of glutamate to GluN2B’s LBD and glycine or D-serine to GluN1, along with membrane depolarization to relieve the Mg²⁺ block. This ligand binding induces LBD clamshell closure, transmitting conformational changes through the TMD to open the channel and permit Na⁺, K⁺, and especially Ca²⁺ influx. GluN2B-containing receptors are especially prominent during early neurodevelopment and at extrasynaptic sites, where they enable synaptic plasticity processes such as long-term potentiation (LTP). Phosphorylation of GluN2B at Tyr1472 by Src family kinases enhances Ca²⁺ permeability and couples receptor activation to CaMKII-mediated spine remodeling and memory formation. While GluN2B-driven signaling is essential for neurodevelopment and learning, its overactivation is implicated in excitotoxic neurodegeneration, such as in Alzheimer’s, Parkinson’s, stroke, and chronic pain, where excessive Ca²⁺ entry triggers cell death pathways.

References
https://pubmed.ncbi.nlm.nih.gov/37324591/ https://pubmed.ncbi.nlm.nih.gov/30037851/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%