NFAT1 Antibody [L23B6] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
WB1:2000 IHC1:50 - 1:200 IF1:100 - 1:1000 FCM1:1000

Application
WB, IHC, IF, FCM

Reactivity
Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
100 kDa 150 kDa,62 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human spleen tissue; Human colon carcinoma tissues; Human tonsil; Jurkat cells; HeLa cells; HeLa cells (staurosporine, 1uM, 3 h); U251 Cells; MCF-7 cells
Negative Control	HeLa cells

Datasheet & SDS

Biological Description

Specificity
NFAT1 Antibody [L23B6] detects endogenous levels of total NFAT1 protein.

Clone
L23B6

Synonym(s)
NFAT1; NFATP; NFATC2; NF-ATc2; NFATc2; NFAT pre-existing subunit; T-cell transcription factor NFAT1; NF-ATp

Background
NFAT1, also known as Nuclear Factor of Activated T-cells 1 or NFATc2, is a transcription factor from the NFAT family (NFAT1-5) predominantly found in immune cells such as T lymphocytes, as well as in neuronal tissues. It regulates gene expression in response to calcium signaling. NFAT1 contains an intrinsically disordered N-terminal regulatory domain with serine-rich regions for phosphorylation, a central Rel-homology DNA-binding domain with an immunoglobulin-like fold that recognizes GGAAA consensus sequences, and a C-terminal transactivation domain that enables cooperative interactions with partners like AP-1 and GATA. In its resting state, NFAT1 remains hyperphosphorylated in the cytoplasm. Upon antigen receptor engagement or calcium influx, the phosphatase calcineurin dephosphorylates NFAT1 at key serine residues, exposing nuclear localization signals. This allows NFAT1 to translocate to the nucleus, bind DNA, and activate transcription of genes such as IL-2 and TNF-α. NFAT1 is central to the Ca2+/calcineurin-NFAT signaling pathway, driving T-cell activation, Th1/Th2 differentiation, cytokine production, and T-cell proliferation and differentiation. In neurons, NFAT1 contributes to axon growth and survival. NFAT1 also forms complexes with AP-1 (c-Fos/Jun) on composite promoter elements, allowing for fine-tuned control of immune responses. Deficiency in NFAT1 leads to hyperproliferation, autoimmunity, and reduced IL-2 expression in animal models. Dysregulated NFAT1 activity is implicated in autoimmune diseases such as rheumatoid arthritis due to excessive cytokine production, and in cancers like lymphomas and diffuse large B-cell lymphoma, where it promotes proliferation through cyclin deregulation.

Background

NFAT1, also known as Nuclear Factor of Activated T-cells 1 or NFATc2, is a transcription factor from the NFAT family (NFAT1-5) predominantly found in immune cells such as T lymphocytes, as well as in neuronal tissues. It regulates gene expression in response to calcium signaling. NFAT1 contains an intrinsically disordered N-terminal regulatory domain with serine-rich regions for phosphorylation, a central Rel-homology DNA-binding domain with an immunoglobulin-like fold that recognizes GGAAA consensus sequences, and a C-terminal transactivation domain that enables cooperative interactions with partners like AP-1 and GATA. In its resting state, NFAT1 remains hyperphosphorylated in the cytoplasm. Upon antigen receptor engagement or calcium influx, the phosphatase calcineurin dephosphorylates NFAT1 at key serine residues, exposing nuclear localization signals. This allows NFAT1 to translocate to the nucleus, bind DNA, and activate transcription of genes such as IL-2 and TNF-α. NFAT1 is central to the Ca2+/calcineurin-NFAT signaling pathway, driving T-cell activation, Th1/Th2 differentiation, cytokine production, and T-cell proliferation and differentiation. In neurons, NFAT1 contributes to axon growth and survival. NFAT1 also forms complexes with AP-1 (c-Fos/Jun) on composite promoter elements, allowing for fine-tuned control of immune responses. Deficiency in NFAT1 leads to hyperproliferation, autoimmunity, and reduced IL-2 expression in animal models. Dysregulated NFAT1 activity is implicated in autoimmune diseases such as rheumatoid arthritis due to excessive cytokine production, and in cancers like lymphomas and diffuse large B-cell lymphoma, where it promotes proliferation through cyclin deregulation.

References
https://pubmed.ncbi.nlm.nih.gov/27399331/ https://pubmed.ncbi.nlm.nih.gov/11877454/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%