NEDD8 Antibody [F18P17] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:50 IHC1:300

Application
WB, IP, IHC

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
9 kDa

Positive Control	Human colon carcinoma; Human ovarian carcinoma; Human breast carcinoma; HeLa cells; RAW cells; C6 cells; COS cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
NEDD8 Antibody [F18P17] detects endogenous levels of total NEDD8 protein.

Clone
F18P17

Synonym(s)
Ubiquitin-like protein NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8 (NEDD-8); NEDD8

Background
NEDD8, or Neural precursor cell expressed developmentally downregulated protein 8, is a ubiquitin-like protein of 76 amino acids and a homolog of Rub1. It adopts a compact ubiquitin-fold beta-grasp structure composed of an alpha-helix, a four-stranded beta-sheet, and an exposed C-terminal glycine residue that is crucial for covalent attachment to lysine side chains, most commonly lysine 60 on cullin proteins. This conjugation occurs after maturation by NEDP1 protease cleavage of its precursor. NEDD8 mediates neddylation, a reversible post-translational modification that activates cullin-RING ligase (CRL) E3 ubiquitin complexes. Neddylation is catalyzed sequentially by E1 (NAE1/UBA3), E2 (UBC12), and E3 (RBX1) enzymes, leading to enhanced cullin dimerization, improved recruitment of E2-ubiquitin conjugates, and increased ubiquitination activity towards substrates such as cyclin E, p27, HIF1 alpha, and NF-kappaB regulators. Through these effects, NEDD8 drives vital processes including cell cycle progression, DNA repair, inflammation, and stress responses. Deneddylation by the COP9 signalosome (CSN) and USP21 recycles cullins and maintains cellular homeostasis. Dysregulated neddylation contributes to diseases such as cancer, where NAE1 overexpression in lung and hepatocellular carcinomas leads to CRL hyperactivation and tumor growth, as well as to neurodegeneration and viral infections.

Background

NEDD8, or Neural precursor cell expressed developmentally downregulated protein 8, is a ubiquitin-like protein of 76 amino acids and a homolog of Rub1. It adopts a compact ubiquitin-fold beta-grasp structure composed of an alpha-helix, a four-stranded beta-sheet, and an exposed C-terminal glycine residue that is crucial for covalent attachment to lysine side chains, most commonly lysine 60 on cullin proteins. This conjugation occurs after maturation by NEDP1 protease cleavage of its precursor. NEDD8 mediates neddylation, a reversible post-translational modification that activates cullin-RING ligase (CRL) E3 ubiquitin complexes. Neddylation is catalyzed sequentially by E1 (NAE1/UBA3), E2 (UBC12), and E3 (RBX1) enzymes, leading to enhanced cullin dimerization, improved recruitment of E2-ubiquitin conjugates, and increased ubiquitination activity towards substrates such as cyclin E, p27, HIF1 alpha, and NF-kappaB regulators. Through these effects, NEDD8 drives vital processes including cell cycle progression, DNA repair, inflammation, and stress responses. Deneddylation by the COP9 signalosome (CSN) and USP21 recycles cullins and maintains cellular homeostasis. Dysregulated neddylation contributes to diseases such as cancer, where NAE1 overexpression in lung and hepatocellular carcinomas leads to CRL hyperactivation and tumor growth, as well as to neurodegeneration and viral infections.

References
https://pubmed.ncbi.nlm.nih.gov/21779466/ https://pubmed.ncbi.nlm.nih.gov/18802447/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%