Mouse IgG Antibody [B6P15] | Primary Antibodies

Application: Reactivity: Mouse

Usage Information

Dilution
WB1:2000-1:10000

Application
WB, ELISA

Reactivity
Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
38 kDa

Datasheet & SDS

Biological Description

Specificity
Mouse IgG Antibody [B6P15] detects endogenous levels of total IgG protein.

Clone
B6P15

Synonym(s)
Constant region of heavy chain of IgG1; G1m marker; Gm marker; Ig gamma 1 chain C region; Immunoglobin heavy constant gamma 1; Immunoglobulin gamma 1

Background
Mouse IgG is the predominant immunoglobulin isotype in mouse serum, with four subclasses, IgG1, IgG2a, IgG2b, and IgG3, produced by plasma B cells as part of the humoral immune response. Each IgG molecule consists of two identical γ heavy chains and two light chains (κ or λ), forming a characteristic Y-shaped structure with antigen-binding Fab regions linked via a flexible, proline- and cysteine-rich hinge to the Fc region (CH2-CH3 domains with conserved N-glycosylation). Subclass differences in hinge length and disulfide bonding influence molecular flexibility and affinity for Fcγ receptors (FcγRI–III), which in turn dictate effector functions. Mouse IgG neutralizes pathogens by antigen binding, opsonizes targets via complement activation (notably IgG2a and IgG2b), and mediates antibody-dependent cellular cytotoxicity (ADCC) through distinct FcγR interactions: IgG2a is highly effective at both complement activation and ADCC, IgG1 is associated with Th2 responses and modest effector activity, IgG3 provides strong opsonization, and IgG2b balances phagocytosis and cytotoxicity. These functions drive cytokine production (such as IFNγ and IL-4), promote immune complex clearance, establish B cell memory, and regulate innate immune responses through neonatal FcRn-mediated recycling that extends serum half-life. Subclass imbalances are implicated in autoimmune diseases (e.g., IgG2a in lupus models), allergies (IgG1), and antitumor immunity, while conserved Fc glycosylation patterns further modulate inflammatory potential.

Background

Mouse IgG is the predominant immunoglobulin isotype in mouse serum, with four subclasses, IgG1, IgG2a, IgG2b, and IgG3, produced by plasma B cells as part of the humoral immune response. Each IgG molecule consists of two identical γ heavy chains and two light chains (κ or λ), forming a characteristic Y-shaped structure with antigen-binding Fab regions linked via a flexible, proline- and cysteine-rich hinge to the Fc region (CH2-CH3 domains with conserved N-glycosylation). Subclass differences in hinge length and disulfide bonding influence molecular flexibility and affinity for Fcγ receptors (FcγRI–III), which in turn dictate effector functions. Mouse IgG neutralizes pathogens by antigen binding, opsonizes targets via complement activation (notably IgG2a and IgG2b), and mediates antibody-dependent cellular cytotoxicity (ADCC) through distinct FcγR interactions: IgG2a is highly effective at both complement activation and ADCC, IgG1 is associated with Th2 responses and modest effector activity, IgG3 provides strong opsonization, and IgG2b balances phagocytosis and cytotoxicity. These functions drive cytokine production (such as IFNγ and IL-4), promote immune complex clearance, establish B cell memory, and regulate innate immune responses through neonatal FcRn-mediated recycling that extends serum half-life. Subclass imbalances are implicated in autoimmune diseases (e.g., IgG2a in lupus models), allergies (IgG1), and antitumor immunity, while conserved Fc glycosylation patterns further modulate inflammatory potential.

References
https://pubmed.ncbi.nlm.nih.gov/25368619/ https://pubmed.ncbi.nlm.nih.gov/18064051/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%