mGluR5 Antibody [D5P24] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:50 IF1:200

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
132 kDa 300 kDa, 150 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Datasheet & SDS

Biological Description

Specificity
mGluR5 Antibody [D5P24] detects endogenous levels of total mGluR5 protein.

Clone
D5P24

Synonym(s)
glutamate metabotropic receptor 5, glutamate receptor, metabotropic 5, GPRC1E, GRM5, Metabotropic glutamate receptor 5, mGlu5, MGLUR5, PPP1R86, protein phosphatase 1, regulatory subunit 86

Background
Metabotropic glutamate receptor 5 (mGluR5) is a group I class C G protein‑coupled glutamate receptor that belongs to the GRM1/GRM5 subfamily and couples primarily to Gq/11 to regulate excitatory synaptic transmission and plasticity in the central nervous system. The receptor contains a large bilobed extracellular Venus flytrap domain that binds glutamate, a cysteine‑rich linker, a seven‑transmembrane helical core, and an intracellular C‑terminal tail that associates with scaffold and signaling proteins at postsynaptic densities and at intracellular membranes. Activation by glutamate induces conformational changes that promote Gq/11‑dependent stimulation of phospholipase C, generation of inositol 1,4,5‑trisphosphate and diacylglycerol, release of Ca²⁺ from intracellular stores, and activation of protein kinase C, with additional engagement of mitogen‑activated protein kinase cascades and protein kinase D phosphorylation that extends the temporal range of mGluR5 signaling. The receptor localizes at perisynaptic regions of excitatory synapses where it interacts functionally with NMDA receptor signaling to modulate synaptic strength, dendritic excitability, and long‑term forms of synaptic plasticity, and intracellular pools of mGluR5 on endomembranes and nuclear compartments couple to distinct Gq/11‑linked pathways that generate localized Ca²⁺ signals and transcriptional responses associated with sustained synaptic activity. mGluR5 is expressed at high levels in cortical, hippocampal, and striatal neurons and is also detectable in astrocytes and microglia, where receptor activation influences glutamate handling, neuroglial signaling, and the balance between pro‑ and anti‑inflammatory outputs, placing this receptor at the interface of neuronal and glial regulation of circuit excitability. The C‑terminal domain forms complexes with PSD‑95, Homer, and SHANK family proteins that organize receptor positioning in perisynaptic nanodomains and couple mGluR5 activation to downstream effectors controlling spine morphology, receptor trafficking, and gene expression programs linked to learning and memory and to stress‑related behavioral responses. Dysregulated mGluR5 signaling associates with fragile X‑related synaptic dysfunction, addiction, anxiety, mood disorders, neurodegeneration, and neuropathic pain, where altered receptor expression or signaling bias contributes to changes in synaptic plasticity, nociceptive processing, or emotional behavior.

Background

Metabotropic glutamate receptor 5 (mGluR5) is a group I class C G protein‑coupled glutamate receptor that belongs to the GRM1/GRM5 subfamily and couples primarily to Gq/11 to regulate excitatory synaptic transmission and plasticity in the central nervous system. The receptor contains a large bilobed extracellular Venus flytrap domain that binds glutamate, a cysteine‑rich linker, a seven‑transmembrane helical core, and an intracellular C‑terminal tail that associates with scaffold and signaling proteins at postsynaptic densities and at intracellular membranes. Activation by glutamate induces conformational changes that promote Gq/11‑dependent stimulation of phospholipase C, generation of inositol 1,4,5‑trisphosphate and diacylglycerol, release of Ca²⁺ from intracellular stores, and activation of protein kinase C, with additional engagement of mitogen‑activated protein kinase cascades and protein kinase D phosphorylation that extends the temporal range of mGluR5 signaling. The receptor localizes at perisynaptic regions of excitatory synapses where it interacts functionally with NMDA receptor signaling to modulate synaptic strength, dendritic excitability, and long‑term forms of synaptic plasticity, and intracellular pools of mGluR5 on endomembranes and nuclear compartments couple to distinct Gq/11‑linked pathways that generate localized Ca²⁺ signals and transcriptional responses associated with sustained synaptic activity. mGluR5 is expressed at high levels in cortical, hippocampal, and striatal neurons and is also detectable in astrocytes and microglia, where receptor activation influences glutamate handling, neuroglial signaling, and the balance between pro‑ and anti‑inflammatory outputs, placing this receptor at the interface of neuronal and glial regulation of circuit excitability. The C‑terminal domain forms complexes with PSD‑95, Homer, and SHANK family proteins that organize receptor positioning in perisynaptic nanodomains and couple mGluR5 activation to downstream effectors controlling spine morphology, receptor trafficking, and gene expression programs linked to learning and memory and to stress‑related behavioral responses. Dysregulated mGluR5 signaling associates with fragile X‑related synaptic dysfunction, addiction, anxiety, mood disorders, neurodegeneration, and neuropathic pain, where altered receptor expression or signaling bias contributes to changes in synaptic plasticity, nociceptive processing, or emotional behavior.

References
https://pubmed.ncbi.nlm.nih.gov/11880516/ https://pubmed.ncbi.nlm.nih.gov/23205012/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%