ME2 Antibody [B19K5] | Primary Antibodies

Application: Reactivity: Human

Lane 1: Jurkat, Lane 2: K562, Lane 3: HL60, Lane 4: 293T

Usage Information

Dilution
WB1:1000-1:10000 IHC1:50-1:100 FCM1:100

Application
WB, IHC, IF, FCM

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
65 kDa

Datasheet & SDS

Biological Description

Specificity
ME2 Antibody [B19K5] detects endogenous levels of total ME2 protein.

Clone
B19K5

Synonym(s)
NAD-ME; Malic enzyme 2; ME2

Background
Mitochondrial malic enzyme 2 (ME2) is a NAD(P)‑dependent dehydrogenase that belongs to the mitochondrial malic enzyme family and is expressed in tissues with high metabolic demand, such as the liver, skeletal muscle, and pancreatic islets. It localizes to the mitochondrial matrix, where it catalyzes the oxidative decarboxylation of malate to pyruvate, generating NAD(P)H and CO₂ and thereby linking the tricarboxylic acid cycle to cytosolic pathways that depend on reducing equivalents. ME2 activity is regulated by key metabolites, including activation by fumarate and inhibition by ATP, which couples its catalytic output to the energetic state of the cell and ensures that NAD(P)H production aligns with mitochondrial capacity and substrate availability. Through the malate‑aspartate shuttle and related metabolite exchanges, ME2 participates in the transfer of reducing equivalents between the cytosol and mitochondria, supporting respiration, ATP synthesis, and the maintenance of redox balance. ME2 also contributes to glucose‑induced insulin secretion by modulating the flux of carbon intermediates and redox cofactors that influence the mitochondrial ATP/ADP ratio and downstream signaling to exocytosis. ME2‑mediated NADPH generation can also impact biosynthetic pathways and redox‑sensitive processes, including aspects of lipid metabolism and stress‑response signaling, which has led to its implication in metabolic dysregulation associated with diabetes and related metabolic disorders.

Background

Mitochondrial malic enzyme 2 (ME2) is a NAD(P)‑dependent dehydrogenase that belongs to the mitochondrial malic enzyme family and is expressed in tissues with high metabolic demand, such as the liver, skeletal muscle, and pancreatic islets. It localizes to the mitochondrial matrix, where it catalyzes the oxidative decarboxylation of malate to pyruvate, generating NAD(P)H and CO₂ and thereby linking the tricarboxylic acid cycle to cytosolic pathways that depend on reducing equivalents. ME2 activity is regulated by key metabolites, including activation by fumarate and inhibition by ATP, which couples its catalytic output to the energetic state of the cell and ensures that NAD(P)H production aligns with mitochondrial capacity and substrate availability. Through the malate‑aspartate shuttle and related metabolite exchanges, ME2 participates in the transfer of reducing equivalents between the cytosol and mitochondria, supporting respiration, ATP synthesis, and the maintenance of redox balance. ME2 also contributes to glucose‑induced insulin secretion by modulating the flux of carbon intermediates and redox cofactors that influence the mitochondrial ATP/ADP ratio and downstream signaling to exocytosis. ME2‑mediated NADPH generation can also impact biosynthetic pathways and redox‑sensitive processes, including aspects of lipid metabolism and stress‑response signaling, which has led to its implication in metabolic dysregulation associated with diabetes and related metabolic disorders.

References
https://pubmed.ncbi.nlm.nih.gov/35435229/ https://pubmed.ncbi.nlm.nih.gov/19691144/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%