MBP tag Antibody [E2A2] | Primary Antibodies

Application: Reactivity: All

Lane 1: Recombinant protein

Usage Information

Dilution
WB1:1000-1:8000 IF1:500-1:2000

Application
WB, IP, IF, ELISA

Reactivity
All

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
40 kDa 40 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	HEK-293T cells (MBP-tag transfected)
Negative Control

Datasheet & SDS

Biological Description

Specificity
MBP tag Antibody [E2A2] detects exogenous levels of total MBP tag protein.

Clone
E2A2

Synonym(s)
MBP; Maltose Binding Protein

Background
The MBP tag (Maltose Binding Protein) is a leading affinity and solubility tag derived from the E. coli MalE gene, widely used to enhance recombinant protein expression by inhibiting inclusion body formation through chaperone-like activity that promotes proper folding of aggregation-prone proteins. It enables efficient, one-step purification by binding with high affinity to amylose resin and can be gently eluted with maltose under native conditions. Mature monomeric MBP consists of two globular domains joined by a flexible hinge, undergoing a conformational switch from open (apo) to closed (holo) upon maltose binding within a deep interdomain groove, with a conserved epitope allowing for specific anti-MBP antibody detection and forming the structural basis for amylose recognition. MBP fusions significantly boost cytoplasmic expression yields in E. coli, confer protease protection, aid in protein crystallization for structural biology, and are commonly engineered with Factor Xa or TEV protease cleavage sites and flexible linkers for precise tag removal after purification.

Background

The MBP tag (Maltose Binding Protein) is a leading affinity and solubility tag derived from the E. coli MalE gene, widely used to enhance recombinant protein expression by inhibiting inclusion body formation through chaperone-like activity that promotes proper folding of aggregation-prone proteins. It enables efficient, one-step purification by binding with high affinity to amylose resin and can be gently eluted with maltose under native conditions. Mature monomeric MBP consists of two globular domains joined by a flexible hinge, undergoing a conformational switch from open (apo) to closed (holo) upon maltose binding within a deep interdomain groove, with a conserved epitope allowing for specific anti-MBP antibody detection and forming the structural basis for amylose recognition. MBP fusions significantly boost cytoplasmic expression yields in E. coli, confer protease protection, aid in protein crystallization for structural biology, and are commonly engineered with Factor Xa or TEV protease cleavage sites and flexible linkers for precise tag removal after purification.

References
https://pubmed.ncbi.nlm.nih.gov/27029048/ https://pubmed.ncbi.nlm.nih.gov/26682969/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%