Home Primary Antibodies Mannose Receptor Antibody [E13A19]

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Mannose Receptor Antibody [E13A19]

Catalog No.: F4779

    Application: Reactivity:
    • F4779-wb
      Lane 1: Mouse liver, Lane 2: Rat liver, Lane 3: Mouse spleen

    Usage Information

    Dilution
    1:1000
    1:400
    1:100
    Application
    WB, IHC, IF
    Reactivity
    Mouse, Rat
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    166 kDa 200 kDa, 165 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.
    Positive Control Rat liver tissue; Mouse liver tissue; Mouse lung cancer tissue; Mouse spleen tissue; Rat spleen tissue; Mouse spleen tissue; Mouse lung tissue; Rat lung tissue; Raw264.7 cell (IL-4, 20ng/ml; Dexamethasone, 10uM, 18 h); Raw 264.7 cell (IL-4, 40ng/ml, 4 d; IL-10, 40ng/ml, 4 d)
    Negative Control Rat brain tissue; Mouse brain tissue; Raw 264.7 cell

    Datasheet & SDS

    Biological Description

    Specificity
    Mannose Receptor Antibody [E13A19] detects endogenous levels of total Mannose Receptor protein.
    Clone
    E13A19
    Synonym(s)
    CD206; Macrophage mannose receptor 1; MMR; Mrc1
    Background
    The Mannose Receptor (MR, CD206, MRC1) is a highly glycosylated type I transmembrane protein of the C-type lectin receptor family, predominantly expressed on macrophages, dendritic cells, and hepatic sinusoidal endothelial cells. It comprises an N-terminal cysteine-rich domain that binds sulfated glycans, a fibronectin type II domain for collagen recognition, and eight contiguous C-type lectin-like domains (CTLDs 1–8) that recognize mannose, fucose, and N-acetylglucosamine on pathogens and endogenous glycoproteins, followed by a single transmembrane helix and a short cytoplasmic tail containing a tyrosine-based internalization motif but lacking classical signaling domains. The Mannose Receptor undergoes clathrin-mediated endocytosis and recycles between the plasma membrane and early endosomes, directing bound ligands to lysosomes for degradation. It plays a key role in pathogen clearance through carbohydrate-specific endocytosis and phagocytosis, facilitates antigen processing and cross-presentation to T cells to initiate adaptive immunity, and removes endogenous glycoproteins to maintain homeostasis. High MR expression characterizes M2-polarized tumor-associated macrophages that suppress anti-tumor immunity, and dysregulation of MR is implicated in tuberculosis persistence and cancer progression.
    References
    • https://pubmed.ncbi.nlm.nih.gov/24672807/
    • https://pubmed.ncbi.nlm.nih.gov/34721436/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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