Lysozyme Antibody [D18K12] | Primary Antibodies

Application: Reactivity: Mouse, Human

Lane 1: RAW 264.7, Lane 2: THP-1

Usage Information

Dilution
WB1:10000-1:50000 IHC1:1000-1:5000 IF1:250

Application
WB, IHC, IF

Reactivity
Mouse, Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
16 kDa 10, 90-110 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Datasheet & SDS

Biological Description

Specificity
Lysozyme Antibody [D18K12] detects endogenous levels of total Lysozyme protein.

Clone
D18K12

Synonym(s)
LZM; LYZ; Lysozyme C

Background
Lysozyme (muramidase), a ubiquitous glycoside hydrolase family 22 enzyme secreted across mucosal surfaces, granules, and exocrine glands, executes non-lytic bactericidal activity by catalyzing hydrolysis of β-1,4 glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine in Gram-positive peptidoglycan, creating protoplasts vulnerable to osmotic lysis. Its canonical α/α barrel fold cradles two glutamates and aspartates in the active site cleft, where Glu35 acts as general acid to protonate the scissile oxygen while Asp52 stabilizes the oxocarbenium ion intermediate, with rigid substrate positioning achieved through extensive hydrogen bonding to acetamido groups. Beyond canonical hydrolysis, lysozyme synergizes with lactoferrin-mediated iron sequestration and defensin pore formation to dismantle Gram-negative outer membranes through synergistic peptidoglycan exposure, while contributing to biofilm disruption via matrix polysaccharide degradation in chronic infections. Secreted by Paneth cells, neutrophils, and acinar cells under NF-κB and STAT3 transcriptional control during bacterial challenge, lysozyme maintains sterile barriers at gut, respiratory, and ocular surfaces, with constitutive tears/saliva levels providing immediate pathogen surveillance. Researchers exploit its heat stability and chromogenic substrate assays for real-time innate immune monitoring in organoid infection models or quantifying granulocyte degranulation via ELISA. Elevated serum levels serve as sarcoidosis activity biomarkers due to macrophage hypersecretion, while amyloidogenic mutations trigger hereditary renal amyloidosis through fibril deposition.

Background

Lysozyme (muramidase), a ubiquitous glycoside hydrolase family 22 enzyme secreted across mucosal surfaces, granules, and exocrine glands, executes non-lytic bactericidal activity by catalyzing hydrolysis of β-1,4 glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine in Gram-positive peptidoglycan, creating protoplasts vulnerable to osmotic lysis. Its canonical α/α barrel fold cradles two glutamates and aspartates in the active site cleft, where Glu35 acts as general acid to protonate the scissile oxygen while Asp52 stabilizes the oxocarbenium ion intermediate, with rigid substrate positioning achieved through extensive hydrogen bonding to acetamido groups. Beyond canonical hydrolysis, lysozyme synergizes with lactoferrin-mediated iron sequestration and defensin pore formation to dismantle Gram-negative outer membranes through synergistic peptidoglycan exposure, while contributing to biofilm disruption via matrix polysaccharide degradation in chronic infections. Secreted by Paneth cells, neutrophils, and acinar cells under NF-κB and STAT3 transcriptional control during bacterial challenge, lysozyme maintains sterile barriers at gut, respiratory, and ocular surfaces, with constitutive tears/saliva levels providing immediate pathogen surveillance. Researchers exploit its heat stability and chromogenic substrate assays for real-time innate immune monitoring in organoid infection models or quantifying granulocyte degranulation via ELISA. Elevated serum levels serve as sarcoidosis activity biomarkers due to macrophage hypersecretion, while amyloidogenic mutations trigger hereditary renal amyloidosis through fibril deposition.

References
https://pubmed.ncbi.nlm.nih.gov/28049649/ https://pubmed.ncbi.nlm.nih.gov/33645532/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%