LCP1 Antibody [P20F21] | Primary Antibodies

Application: Reactivity: Human, Mouse

Lane 1: Ramos, Lane 2: Raji

Usage Information

Dilution
WB1:1000 IHC1:400

Application
WB, IHC

Reactivity
Human, Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
70 kDa

Datasheet & SDS

Biological Description

Specificity
LCP1 Antibody [P20F21] detects endogenous levels of total LCP1 protein.

Clone
P20F21

Synonym(s)
Plastin-2; L-plastin; LC64P; Lymphocyte cytosolic protein 1; LCP1

Background
LCP1 belongs to the plastin family of actin-bundling proteins alongside T-plastin and I-plastin, with expression concentrated in hematopoietic lineages and epithelial-derived malignancies where it stabilizes F-actin networks essential for cytoskeletal dynamics. LCP1 organizes two calponin-homology actin-binding domains connected by a flexible linker and flanked by regulatory headpiece and coiled-coil motifs that enable cross-linking of actin filaments into parallel bundles while maintaining responsiveness to phosphorylation signals. Upon T cell receptor engagement with CD3 and costimulatory inputs from CD2 or CD28, Lck-mediated phosphorylation at Ser5 within the headpiece domain relieves autoinhibition, enhancing affinity for F-actin barbed ends and promoting lamellipodial protrusion through Arp2/3-independent bundling that couples to integrin-mediated adhesion maturation. This activation integrates with PI3K-AKT signaling to amplify Rac1/Cdc42 guanine nucleotide exchange, driving polarized actin flow and uropod retraction during lymphocyte transmigration across endothelial barriers, while in monocytes it coordinates phagocytic cup formation via WASP-Arp2/3 synergy at pathogen contact sites. LCP1 further scaffolds cortactin and cortactins to focal contacts, facilitating FAK-Src cascade propagation that sustains mechanotransduction and matrix degradation through MMP9 secretion in invasive fronts. LCP1 governs immune synapse assembly and chemotactic haptotaxis in dendritic cells patrolling lymphoid tissues, with peak activity in circulating leukocytes reflecting rapid on-off cycling tuned by protein kinase C theta. Elevated LCP1 drives metastatic dissemination in colorectal, ovarian, and osteosarcoma lesions through JAK2-STAT3-mediated epithelial-mesenchymal transition and exosomal transfer that primes distant niches.

Background

LCP1 belongs to the plastin family of actin-bundling proteins alongside T-plastin and I-plastin, with expression concentrated in hematopoietic lineages and epithelial-derived malignancies where it stabilizes F-actin networks essential for cytoskeletal dynamics. LCP1 organizes two calponin-homology actin-binding domains connected by a flexible linker and flanked by regulatory headpiece and coiled-coil motifs that enable cross-linking of actin filaments into parallel bundles while maintaining responsiveness to phosphorylation signals. Upon T cell receptor engagement with CD3 and costimulatory inputs from CD2 or CD28, Lck-mediated phosphorylation at Ser5 within the headpiece domain relieves autoinhibition, enhancing affinity for F-actin barbed ends and promoting lamellipodial protrusion through Arp2/3-independent bundling that couples to integrin-mediated adhesion maturation. This activation integrates with PI3K-AKT signaling to amplify Rac1/Cdc42 guanine nucleotide exchange, driving polarized actin flow and uropod retraction during lymphocyte transmigration across endothelial barriers, while in monocytes it coordinates phagocytic cup formation via WASP-Arp2/3 synergy at pathogen contact sites. LCP1 further scaffolds cortactin and cortactins to focal contacts, facilitating FAK-Src cascade propagation that sustains mechanotransduction and matrix degradation through MMP9 secretion in invasive fronts. LCP1 governs immune synapse assembly and chemotactic haptotaxis in dendritic cells patrolling lymphoid tissues, with peak activity in circulating leukocytes reflecting rapid on-off cycling tuned by protein kinase C theta. Elevated LCP1 drives metastatic dissemination in colorectal, ovarian, and osteosarcoma lesions through JAK2-STAT3-mediated epithelial-mesenchymal transition and exosomal transfer that primes distant niches.

References
https://pubmed.ncbi.nlm.nih.gov/22194750/ https://pubmed.ncbi.nlm.nih.gov/39588922/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%