LAG3 Antibody [K23N22]

Catalog No.: F2211

For research use only.

    Home Primary Antibodies LAG3 Antibody [K23N22]

    Application: Reactivity:

    Usage Information

    Dilution
    1:500
    1:30
    1:1000
    1:100
    1:500
    Application
    WB, IP, IHC, IF, FCM
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    54kDa 54-70 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    LAG3 Antibody [K23N22] detects endogenous levels of total LAG3 protein.
    Clone
    K23N22
    Synonym(s)
    CD223; FDC; LAG3; Lymphocyte activation gene 3 protein; LAG-3
    Background
    LAG3 (Lymphocyte activation gene 3, CD223) is a type I transmembrane inhibitory receptor of the immunoglobulin superfamily that functions as an immune checkpoint, structurally related to CD4 but with distinct regulatory features. It is encoded on chromosome 12p13.32 in humans, producing a ~70 kDa protein composed of four extracellular Ig-like domains (D1–D4), a connecting peptide, a single transmembrane domain, and a short cytoplasmic tail containing three conserved motifs (a serine phosphorylation site, KIEELE, and EP repeats) that mediate inhibitory signaling. LAG3 is predominantly expressed on activated CD4⁺ and CD8⁺ T cells, regulatory T cells, natural killer (NK) cells, B cells, and plasmacytoid dendritic cells, with expression strongly upregulated upon chronic antigen stimulation, particularly in cancer and chronic infections. Functionally, LAG3 binds MHC class II with higher affinity than CD4 and also interacts with other ligands such as fibrinogen-like protein 1 (FGL1), galectin-3, LSECtin, and the TCR–CD3 complex, leading to suppression of T cell proliferation, cytokine secretion, calcium signaling, and effector functions. By dampening TCR signaling and promoting T cell exhaustion, LAG3 serves as a critical regulator of immune homeostasis and a key immune checkpoint in the tumor microenvironment, making it an important therapeutic target in cancer immunotherapy and autoimmunity.
    References
    • https://pubmed.ncbi.nlm.nih.gov/34067904/
    • https://pubmed.ncbi.nlm.nih.gov/38556085/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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