Home Primary Antibodies Influenza A Virus M2 Protein Antibody [L21L24]

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Influenza A Virus M2 Protein Antibody [L21L24]

Catalog No.: F2238

    Application: Reactivity:

    Usage Information

    Dilution
    1:2000
    Application
    IF
    Reactivity
    Influenza A
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years

    Datasheet & SDS

    Biological Description

    Specificity
    Influenza A Virus M2 Protein Antibody [L21L24] detects exogenous levels of total Influenza A Virus M2 protein.
    Clone
    L21L24
    Synonym(s)
    Influenza A virus matrix protein M2; Matrix protein 2; Membrane ion channel M2; Membrane protein M2; Proton channel protein M2
    Background
    The Influenza A Virus (IAV) M2 protein is a 97-amino acid, integral membrane viroporin essential for the viral life cycle, functioning as a homotetrameric, pH-activated, proton-selective ion channel. Its structure consists of an N-terminal ectodomain (residues 1–24) critical for virion incorporation, a transmembrane domain (residues 25–43) forming the channel core, and a C-terminal domain (residues 44–97) that includes an amphipathic helix and an unstructured tail involved in virus assembly and budding. Key residues, His37 and Trp41, serve as the proton sensor and channel gate, respectively. Upon endosomal acidification during viral entry, M2 mediates proton influx into the virion, facilitating release of viral ribonucleoproteins for replication. Subsequently, M2 maintains optimal pH in the trans-Golgi network to prevent premature hemagglutinin activation, while its C-terminal amphipathic helix induces membrane curvature required for viral budding and scission. M2 activity is regulated by pH and host protein interactions, and although it is a target of antivirals like amantadine, drug resistance is common due to mutations in the transmembrane domain.
    References
    • https://pubmed.ncbi.nlm.nih.gov/19601584/
    • https://pubmed.ncbi.nlm.nih.gov/19590009/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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