Home Primary Antibodies IFN-γ Antibody [A4P20]

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IFN-γ Antibody [A4P20]

Catalog No.: F3018

    Application: Reactivity:
    • F3018-wb
      Lane 1: NK-92, Lane 2: NK-92 (TPA, 80 nM, 5 h; Ionomycin, 3 μM, 5 h; Brefeldin A, 300 ng/mL, 4 h)

    Usage Information

    Dilution
    1:1000
    1:50
    1:100
    1:400
    Application
    WB, IP, IF, FCM
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    17 kDa, 19 kDa, 23 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    IFN-γ Antibody [A4P20] detects endogenous levels of total IFN-γ protein.
    Clone
    A4P20
    Synonym(s)
    Interferon gamma; IFN-gamma; Immune interferon; IFNG
    Background
    Interferon-gamma (IFN-γ) is a type II interferon cytokine that plays a pivotal role in bridging innate and adaptive immunity by activating immune cells such as macrophages and natural killer (NK) cells to enhance their cytotoxic activity. IFN-γ functions by binding to its heterodimeric receptor complex, consisting of IFNGR1 and IFNGR2 subunits, which promotes receptor dimerization and activates the intracellular kinases JAK1 and JAK2. This leads to phosphorylation and activation of STAT1 transcription factors, which translocate to the nucleus to induce the expression of interferon-stimulated genes (ISGs), including antiviral proteins like PKR. IFN-γ enhances antigen presentation by increasing MHC class I and II expression and promotes cell-mediated immunity through the activation of T lymphocytes. It also stimulates macrophages to produce reactive oxygen species, nitric oxide, and inflammatory cytokines, supporting its role in defense against intracellular pathogens such as Chlamydia and Toxoplasma gondii. Dysregulation or deficiency of IFN-γ or its receptor leads to increased susceptibility to infections. Additionally, IFN-γ is involved in inflammatory diseases, including atherosclerosis, and exhibits complex roles in tumor immunity.
    References
    • https://pubmed.ncbi.nlm.nih.gov/14525967/
    • https://pubmed.ncbi.nlm.nih.gov/15864272/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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