Human IgG Antibody [H24A6] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:300 - 1:1000

Application
WB, IHC

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
36 kDa 50 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Positive Control	Human stomach tissue; Human tonsil tissue; Human fetal thymus tissue; Human fetal tonsil tissue; Human fetal spleen tissue; IgG2 recombinant protein; IgG1 recombinant protein; IgG3 recombinant protein; IgG4 recombinant protein
Negative Control

Datasheet & SDS

Biological Description

Specificity
Human IgG Antibody [H24A6] detects endogenous levels of total Human IgG protein.

Clone
H24A6

Synonym(s)
Immunoglobulin heavy constant gamma 1; Ig gamma-1 chain C region; Ig gamma-1 chain C region EU; Ig gamma-1 chain C region KOL; Ig gamma-1 chain C region NIE; IGHG1

Background
Human IgG belongs to the immunoglobulin superfamily as the most abundant antibody isotype in human serum, comprising four subclasses (IgG1-4) produced by plasma cells during humoral immune responses. Each IgG molecule forms a Y-shaped monomer from two identical γ heavy chains and two κ or λ light chains linked by disulfide bonds, with each heavy chain containing one variable domain (VH), three constant domains (CH1, CH2, CH3), and a flexible hinge region between CH1 and CH2. The Fab arms encompass VL/VH and CL/CH1 domains forming antigen-binding sites generated via V(D)J recombination and somatic hypermutation for high-affinity recognition, while the Fc region from CH2-CH3 domains mediates effector functions through interactions with Fcγ receptors and C1q. IgG1 and IgG3 excel at activating complement via classical pathway C1q binding at CH2 residues 318-337, leading to C3b opsonization, membrane attack complex formation, and pathogen lysis. Binding to FcγRI, IIa, or IIIa on NK cells, macrophages, and neutrophils triggers antibody-dependent cellular cytotoxicity (ADCC), releasing perforin/granzyme for target cell elimination, with IgG1 showing highest potency due to extended hinge flexibility. Phagocytes engulf opsonized microbes through FcγRIIa-mediated phagocytosis, enhanced by PI3K signaling for cytoskeletal rearrangement. IgG crosses placenta via neonatal Fc receptor (FcRn) on syncytiotrophoblasts, providing passive immunity to fetuses, while serum half-life extends to 21 days through FcRn recycling in endothelial cells.

Background

Human IgG belongs to the immunoglobulin superfamily as the most abundant antibody isotype in human serum, comprising four subclasses (IgG1-4) produced by plasma cells during humoral immune responses. Each IgG molecule forms a Y-shaped monomer from two identical γ heavy chains and two κ or λ light chains linked by disulfide bonds, with each heavy chain containing one variable domain (VH), three constant domains (CH1, CH2, CH3), and a flexible hinge region between CH1 and CH2. The Fab arms encompass VL/VH and CL/CH1 domains forming antigen-binding sites generated via V(D)J recombination and somatic hypermutation for high-affinity recognition, while the Fc region from CH2-CH3 domains mediates effector functions through interactions with Fcγ receptors and C1q. IgG1 and IgG3 excel at activating complement via classical pathway C1q binding at CH2 residues 318-337, leading to C3b opsonization, membrane attack complex formation, and pathogen lysis. Binding to FcγRI, IIa, or IIIa on NK cells, macrophages, and neutrophils triggers antibody-dependent cellular cytotoxicity (ADCC), releasing perforin/granzyme for target cell elimination, with IgG1 showing highest potency due to extended hinge flexibility. Phagocytes engulf opsonized microbes through FcγRIIa-mediated phagocytosis, enhanced by PI3K signaling for cytoskeletal rearrangement. IgG crosses placenta via neonatal Fc receptor (FcRn) on syncytiotrophoblasts, providing passive immunity to fetuses, while serum half-life extends to 21 days through FcRn recycling in endothelial cells.

References
https://pubmed.ncbi.nlm.nih.gov/14714888/ https://pubmed.ncbi.nlm.nih.gov/26497518/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%