Histone H3 (di/tri methyl K4) Antibody [A19H7]

Application: Reactivity: Cow, Human, Rice

Usage Information

Dilution
WB1:500 IF1:2000 FCM1:2000

Application
WB, IF, FCM

Reactivity
Cow, Human, Rice

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
15 kDa 17 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Datasheet & SDS

Biological Description

Specificity
Histone H3 (di/tri methyl K4) Antibody [A19H7] detects endogenous levels of histone H3 only when it is di- or tri-methylated at lysine 4 (K4).

Clone
A19H7

Synonym(s)
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF

Background
Histone H3 (di/tri methyl K4) refers to histone H3 whose lysine 4 side chain carries two or three methyl groups, a covalent tail modification that functions as a key chromatin signal for transcriptional competence and active promoter architecture. Lys4 lies within the N‑terminal tail of H3 that protrudes from the nucleosome core, where it is accessible to SET1/MLL‑family methyltransferases and KDM‑family demethylases that dynamically install and remove mono‑, di‑, and tri‑methyl states, generating distinct docking surfaces for effector proteins. Dimethylation at H3K4 (H3K4me2) associates broadly with active and poised genes, extending across promoter‑proximal and early gene body regions and marking chromatin that is transcriptionally engaged or readily activatable, whereas trimethylation (H3K4me3) is sharply enriched at transcription start sites of actively transcribed genes and forms a narrow peak that defines active promoter nucleosomes. These methyl marks do not significantly alter nucleosome structure directly but create binding platforms for chromatin readers containing PHD fingers, chromodomains, Tudor domains, and WD40 repeats, including BPTF, ING family proteins, WDR5, and other components of nucleosome remodeling and histone acetyltransferase complexes, which then promote an open chromatin environment and recruitment or stabilization of the transcriptional machinery. H3K4me3 interacts functionally with H3/H4 acetylation and RNA polymerase II pre‑initiation complex assembly, and its presence at promoters correlates with transcription start site nucleosome depletion and high levels of paused and elongating polymerase, integrating it into core promoter logic in development and cell identity. H3K4me2 and H3K4me3 patterns change dynamically during differentiation and in disease, and altered distribution or global levels of these marks are linked to mis‑regulated gene expression programs in cancer, developmental syndromes, and metabolic and inflammatory disorders driven by mutations or aberrant activity of H3K4 methyltransferases and demethylases.

Background

Histone H3 (di/tri methyl K4) refers to histone H3 whose lysine 4 side chain carries two or three methyl groups, a covalent tail modification that functions as a key chromatin signal for transcriptional competence and active promoter architecture. Lys4 lies within the N‑terminal tail of H3 that protrudes from the nucleosome core, where it is accessible to SET1/MLL‑family methyltransferases and KDM‑family demethylases that dynamically install and remove mono‑, di‑, and tri‑methyl states, generating distinct docking surfaces for effector proteins. Dimethylation at H3K4 (H3K4me2) associates broadly with active and poised genes, extending across promoter‑proximal and early gene body regions and marking chromatin that is transcriptionally engaged or readily activatable, whereas trimethylation (H3K4me3) is sharply enriched at transcription start sites of actively transcribed genes and forms a narrow peak that defines active promoter nucleosomes. These methyl marks do not significantly alter nucleosome structure directly but create binding platforms for chromatin readers containing PHD fingers, chromodomains, Tudor domains, and WD40 repeats, including BPTF, ING family proteins, WDR5, and other components of nucleosome remodeling and histone acetyltransferase complexes, which then promote an open chromatin environment and recruitment or stabilization of the transcriptional machinery. H3K4me3 interacts functionally with H3/H4 acetylation and RNA polymerase II pre‑initiation complex assembly, and its presence at promoters correlates with transcription start site nucleosome depletion and high levels of paused and elongating polymerase, integrating it into core promoter logic in development and cell identity. H3K4me2 and H3K4me3 patterns change dynamically during differentiation and in disease, and altered distribution or global levels of these marks are linked to mis‑regulated gene expression programs in cancer, developmental syndromes, and metabolic and inflammatory disorders driven by mutations or aberrant activity of H3K4 methyltransferases and demethylases.

References
https://pubmed.ncbi.nlm.nih.gov/19703438/ https://pubmed.ncbi.nlm.nih.gov/17218268/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%