HDAC11 Antibody [H19H1] | Primary Antibodies

Application: Reactivity: Human

Lane 1: SK-OV-3, Lane 2: T47D

Usage Information

Dilution
WB1:1000-1:6000 IHC1:500-1:2000

Application
WB, IHC, ELISA

Reactivity
Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
39 kDa 39 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Datasheet & SDS

Biological Description

Specificity
HDAC11 Antibody [H19H1] detects endogenous levels of total HDAC11 protein.

Clone
H19H1

Synonym(s)
Histone deacetylase 11; EC:3.5.1.98; HD11; HDAC11

Background
HDAC11 is the only member of the class IV histone deacetylase family, characterized by a compact catalytic domain and limited substrate specificity. It modulates gene expression and immune function in the brain, heart, and immune cells by deacetylating both histone and non-histone proteins. HDAC11 forms repressive complexes with transcription factors such as Egr1 and HEY1, deacetylating them to reduce p53 promoter activity and suppress apoptosis in tumor cells. In antigen-presenting cells, HDAC11 is recruited to IL-10 promoters, silencing this anti-inflammatory cytokine and facilitating T cell priming. Upon innate immune stimulation, HDAC11 integrates into MAPK-NF-κB signaling pathways, directly deacetylating RelA/p65 to sustain inflammatory gene transcription (e.g., TNF-α, IL-6) and coordinating neutrophil maturation via FoxO-dependent granulopoiesis. HDAC11 tempers immune responses by stabilizing FOXP3 in regulatory T cells through indirect deacetylation. This bidirectional control places HDAC11 at the intersection of immune tolerance and activation. Selective inhibitors of HDAC11 can enhance IL-10 expression and restore p53-mediated apoptosis. HDAC11 also regulates emergency myelopoiesis during infection and fine-tunes microglial activity in the CNS. Dysregulation of HDAC11 leads to immune suppression in lymphomas and hepatocellular carcinoma (via p53 silencing) or to hyperinflammation in autoimmune diseases.

Background

HDAC11 is the only member of the class IV histone deacetylase family, characterized by a compact catalytic domain and limited substrate specificity. It modulates gene expression and immune function in the brain, heart, and immune cells by deacetylating both histone and non-histone proteins. HDAC11 forms repressive complexes with transcription factors such as Egr1 and HEY1, deacetylating them to reduce p53 promoter activity and suppress apoptosis in tumor cells. In antigen-presenting cells, HDAC11 is recruited to IL-10 promoters, silencing this anti-inflammatory cytokine and facilitating T cell priming. Upon innate immune stimulation, HDAC11 integrates into MAPK-NF-κB signaling pathways, directly deacetylating RelA/p65 to sustain inflammatory gene transcription (e.g., TNF-α, IL-6) and coordinating neutrophil maturation via FoxO-dependent granulopoiesis. HDAC11 tempers immune responses by stabilizing FOXP3 in regulatory T cells through indirect deacetylation. This bidirectional control places HDAC11 at the intersection of immune tolerance and activation. Selective inhibitors of HDAC11 can enhance IL-10 expression and restore p53-mediated apoptosis. HDAC11 also regulates emergency myelopoiesis during infection and fine-tunes microglial activity in the CNS. Dysregulation of HDAC11 leads to immune suppression in lymphomas and hepatocellular carcinoma (via p53 silencing) or to hyperinflammation in autoimmune diseases.

References
https://pubmed.ncbi.nlm.nih.gov/30899397/ https://ashpublications.org/blood/article/110/11/1330/74078/A-Novel-Role-of-Histone-Deacetylase-11-HDAC11-in

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%