GOPC Antibody [D5A8] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:250 - 1:500 IF1:50 - 1:100

Application
WB, IH, IF

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
51 kDa

Datasheet & SDS

Biological Description

Specificity
GOPC Antibody [D5A8] detects endogenous levels of total GOPC protein.

Clone
D5A8

Synonym(s)
CAL, FIG, GOPC, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST

Background
PIST, also known as GOPC or PDZ domain-containing protein 8, belongs to the family of PDZ domain scaffolding proteins that organize intracellular signaling complexes through specific interactions with the C-terminal tails of transmembrane receptors. Localized primarily at the trans-Golgi network, PIST features a single PDZ domain along with coiled-coil motifs that enable homodimerization and binding to diverse partners such as TC10 GTPase, neuroligin-1, metabotropic glutamate receptor 5 (mGluR5), and β1-adrenergic receptor (β1AR). In biosynthetic trafficking, PIST retains these receptors in the TGN, competing with plasma membrane scaffolds like PSD-95 to modulate their delivery to the cell surface and thereby regulate downstream signaling such as MAPK activation by β1AR agonists. Upon agonist stimulation and cAMP pathway engagement, PIST relocates to endosome-like compartments where it colocalizes with sorting nexin 1 (SNX1) and internalized receptors, stabilizing them against lysosomal degradation during post-endocytic recycling. For synaptic receptors like mGluR5 and neuroligin-1, PIST facilitates plasma membrane targeting in neurons and precise subcellular sorting to postsynaptic densities in hippocampal and cortical regions. Absence of PIST disrupts mGluR5 localization at synapses, leading to impaired metabotropic glutamate receptor-dependent long-term depression and deficits in contextual fear conditioning. Dysregulation manifests in reduced β1AR levels in PIST-deficient mouse hippocampi and altered tight junction protein trafficking, linking it to neurodegenerative disruptions in synaptic organization and potentially broader epithelial barrier functions.

Background

PIST, also known as GOPC or PDZ domain-containing protein 8, belongs to the family of PDZ domain scaffolding proteins that organize intracellular signaling complexes through specific interactions with the C-terminal tails of transmembrane receptors. Localized primarily at the trans-Golgi network, PIST features a single PDZ domain along with coiled-coil motifs that enable homodimerization and binding to diverse partners such as TC10 GTPase, neuroligin-1, metabotropic glutamate receptor 5 (mGluR5), and β1-adrenergic receptor (β1AR). In biosynthetic trafficking, PIST retains these receptors in the TGN, competing with plasma membrane scaffolds like PSD-95 to modulate their delivery to the cell surface and thereby regulate downstream signaling such as MAPK activation by β1AR agonists. Upon agonist stimulation and cAMP pathway engagement, PIST relocates to endosome-like compartments where it colocalizes with sorting nexin 1 (SNX1) and internalized receptors, stabilizing them against lysosomal degradation during post-endocytic recycling. For synaptic receptors like mGluR5 and neuroligin-1, PIST facilitates plasma membrane targeting in neurons and precise subcellular sorting to postsynaptic densities in hippocampal and cortical regions. Absence of PIST disrupts mGluR5 localization at synapses, leading to impaired metabotropic glutamate receptor-dependent long-term depression and deficits in contextual fear conditioning. Dysregulation manifests in reduced β1AR levels in PIST-deficient mouse hippocampi and altered tight junction protein trafficking, linking it to neurodegenerative disruptions in synaptic organization and potentially broader epithelial barrier functions.

References
https://pubmed.ncbi.nlm.nih.gov/34383253/ https://pubmed.ncbi.nlm.nih.gov/25614626/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%