Glutathione Peroxidase 1 Antibody [M21J11]

Application: Reactivity: Human

Lane 1: THP1, Lane 2: THP1 (KO GPX1), Lane 3: HL60, Lane 4: Rat liver

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:100 - 1:250 FCM1:250

Application
WB, IHP, FCM

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
22 kDa 22 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Rat lung tissue; Human breast carcinoma tissue; Human fetal liver tissue; Rat liver tissue; THP-1 cells; HAP1 cells; HL60 cells; HEK-293T cells; SH SY5Y cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
Glutathione Peroxidase 1 Antibody [M21J11] detects endogenous levels of total Glutathione Peroxidase 1 protein.

Clone
M21J11

Synonym(s)
Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; Phospholipid-hydroperoxide glutathione peroxidase GPX1; GPX1

Background
Glutathione Peroxidase 1 (GPx1) is the main intracellular selenoprotein antioxidant enzyme that protects cells from oxidative damage by reducing hydrogen peroxide and lipid hydroperoxides to water and alcohols, using reduced glutathione (GSH) as an electron donor; the resulting oxidized glutathione disulfide (GSSG) is recycled by glutathione reductase. GPx1 is a homotetramer of four identical 22–23 kDa subunits, each encoded by the GPX1 gene (chromosome 3p21.31) and incorporating selenocysteine (Sec) at the active site through UGA codon recoding governed by the SECIS element in the mRNA 3′ UTR. The catalytic tetrad (Sec-Gln-Trp-Asn) resides within a pocket formed by conserved arginine and lysine residues from adjacent subunits, facilitating GSH binding and enabling ping-pong bisubstrate kinetics for efficient peroxide detoxification. Both cytosolic and mitochondrial GPx1 maintain redox homeostasis by working with superoxide dismutase (SOD) and catalase to neutralize reactive oxygen species (ROS), thus preventing lipid peroxidation, protein carbonylation, DNA damage, and aberrant H2O2-mediated signaling that can affect apoptosis, growth factor responses, insulin sensitivity, and Nrf2-dependent gene expression. GPx1 deficiency is linked to cardiovascular diseases (atherosclerosis, endothelial dysfunction), neurodegeneration (as shown by neuroprotection in stroke/traumatic brain injury), and cancer (where its role can be either tumor-promoting or risk-modulating, as influenced by the Pro198Leu polymorphism). GPx1 also serves as a key biomarker of selenium status and is transcriptionally regulated by Nrf2, NF-κB, and p53.

Background

Glutathione Peroxidase 1 (GPx1) is the main intracellular selenoprotein antioxidant enzyme that protects cells from oxidative damage by reducing hydrogen peroxide and lipid hydroperoxides to water and alcohols, using reduced glutathione (GSH) as an electron donor; the resulting oxidized glutathione disulfide (GSSG) is recycled by glutathione reductase. GPx1 is a homotetramer of four identical 22–23 kDa subunits, each encoded by the GPX1 gene (chromosome 3p21.31) and incorporating selenocysteine (Sec) at the active site through UGA codon recoding governed by the SECIS element in the mRNA 3′ UTR. The catalytic tetrad (Sec-Gln-Trp-Asn) resides within a pocket formed by conserved arginine and lysine residues from adjacent subunits, facilitating GSH binding and enabling ping-pong bisubstrate kinetics for efficient peroxide detoxification. Both cytosolic and mitochondrial GPx1 maintain redox homeostasis by working with superoxide dismutase (SOD) and catalase to neutralize reactive oxygen species (ROS), thus preventing lipid peroxidation, protein carbonylation, DNA damage, and aberrant H2O2-mediated signaling that can affect apoptosis, growth factor responses, insulin sensitivity, and Nrf2-dependent gene expression. GPx1 deficiency is linked to cardiovascular diseases (atherosclerosis, endothelial dysfunction), neurodegeneration (as shown by neuroprotection in stroke/traumatic brain injury), and cancer (where its role can be either tumor-promoting or risk-modulating, as influenced by the Pro198Leu polymorphism). GPx1 also serves as a key biomarker of selenium status and is transcriptionally regulated by Nrf2, NF-κB, and p53.

References
https://pubmed.ncbi.nlm.nih.gov/21087145/ https://pubmed.ncbi.nlm.nih.gov/19376195/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%