FTO Antibody [P22L4] | Primary Antibodies

Application: Reactivity: Human

Lane 1: SH-SY5Y, Lane 2: Hela, Lane 3: U87MG

Usage Information

Dilution
WB1:1000 IP1:50 CHIP1:50

Application
WB, IP

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
60 kDa

Datasheet & SDS

Biological Description

Specificity
FTO Antibody [P22L4] detects endogenous levels of total FTO protein.

Clone
P22L4

Synonym(s)
Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO

Background
Fat mass and obesity‑associated protein (FTO) is a member of the Fe²⁺‑ and 2‑oxoglutarate‑dependent dioxygenase family and functions primarily as an RNA N6‑methyladenosine (m6A) and DNA demethylase that fine‑tunes post‑transcriptional and transcriptional programs linked to energy homeostasis and obesity‑associated traits. FTO contains a conserved catalytic double‑stranded β‑helix domain that coordinates iron and 2‑oxoglutarate, allowing it to oxidatively demethylate m6A sites in select mRNA transcripts and 3‑methylthymine in DNA, with its activity modulated by N‑ and C‑terminal regulatory regions that influence subcellular localization and stability. FTO demethylates m6A residues in transcripts involved in dopaminergic signaling, feeding behavior, and energy‑balance regulation, and this erasure generally promotes mRNA stability and translation of key regulators of appetite, reward, and metabolic rate, thereby linking FTO‑dependent methylation dynamics to caloric intake and energy expenditure. FTO deficiency causes postnatal growth retardation, pronounced leanness, and elevated energy expenditure driven by systemic activation of sympathetic neurons, whereas tissue‑specific or systemic overexpression of FTO increases food intake and adiposity and predisposes to obesity, phenotypes that parallel human genetic studies showing that common FTO intronic SNPs associated with higher body mass index correlate with increased food intake and reduced activity. FTO frequently shows altered expression and acts as an oncogenic driver or tumor‑suppressor‑like node depending on cellular context.

Background

Fat mass and obesity‑associated protein (FTO) is a member of the Fe²⁺‑ and 2‑oxoglutarate‑dependent dioxygenase family and functions primarily as an RNA N6‑methyladenosine (m6A) and DNA demethylase that fine‑tunes post‑transcriptional and transcriptional programs linked to energy homeostasis and obesity‑associated traits. FTO contains a conserved catalytic double‑stranded β‑helix domain that coordinates iron and 2‑oxoglutarate, allowing it to oxidatively demethylate m6A sites in select mRNA transcripts and 3‑methylthymine in DNA, with its activity modulated by N‑ and C‑terminal regulatory regions that influence subcellular localization and stability. FTO demethylates m6A residues in transcripts involved in dopaminergic signaling, feeding behavior, and energy‑balance regulation, and this erasure generally promotes mRNA stability and translation of key regulators of appetite, reward, and metabolic rate, thereby linking FTO‑dependent methylation dynamics to caloric intake and energy expenditure. FTO deficiency causes postnatal growth retardation, pronounced leanness, and elevated energy expenditure driven by systemic activation of sympathetic neurons, whereas tissue‑specific or systemic overexpression of FTO increases food intake and adiposity and predisposes to obesity, phenotypes that parallel human genetic studies showing that common FTO intronic SNPs associated with higher body mass index correlate with increased food intake and reduced activity. FTO frequently shows altered expression and acts as an oncogenic driver or tumor‑suppressor‑like node depending on cellular context.

References
https://pubmed.ncbi.nlm.nih.gov/30718435/ https://pubmed.ncbi.nlm.nih.gov/23896822/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%