eIF6 Antibody [A13F7] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:50 IF1:1600

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
27 kDa

Positive Control	293 cells;HepG2 cells;C2C12 cells;KNRK cells;NBTII cells;HeLa cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
eIF6 Antibody [A13F7] detects endogenous levels of total eIF6 protein.

Clone
A13F7

Synonym(s)
Eukaryotic translation initiation factor 6; eIF-6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP); EIF6; EIF3A; ITGB4BP

Background
eIF6 (eukaryotic initiation factor 6, also known as EIF6 or p27BBP1) is a highly conserved nucleolocytoplasmic protein that, despite lacking homology to canonical translation initiation factors, plays a fundamental dual role in ribosome biogenesis and translation control by binding the intersubunit face of free 60S ribosomal subunits. eIF6 adopts a flat, elongated shape with nine α-helices forming two domains that dock into the central protuberance and P-site of the 60S subunit, stabilized by key residues such as Arg133 and Lys151, and requires phosphorylation (notably at Ser174) for its regulated release. eIF6 acts as an anti-association factor, sequestering mature 60S subunits to prevent their premature joining with 40S subunits, thereby maintaining translational fidelity and quality control; in the nucleolus, it facilitates 60S maturation through interactions with SBDS and EFL1, which, via GTP hydrolysis, evict eIF6 and license subunits for translation, thus dynamically coupling ribosome recycling to protein synthesis rates. eIF6 integrates mTOR/S6K signaling to align ribosome availability with cellular growth demands, and associates with RISC to promote miRNA-mediated translational silencing or recycling blocks, its deletion abolishes miRNA repression. eIF6 dysregulation is implicated in Shwachman-Diamond syndrome (where SBDS mutations trap eIF6 and hinder 60S-40S joining), while its overexpression in various cancers (such as colorectal and gastric) drives unrestrained translation and cyclin D1 upregulation, contributing to tumorigenesis and metabolic imbalance.

Background

eIF6 (eukaryotic initiation factor 6, also known as EIF6 or p27BBP1) is a highly conserved nucleolocytoplasmic protein that, despite lacking homology to canonical translation initiation factors, plays a fundamental dual role in ribosome biogenesis and translation control by binding the intersubunit face of free 60S ribosomal subunits. eIF6 adopts a flat, elongated shape with nine α-helices forming two domains that dock into the central protuberance and P-site of the 60S subunit, stabilized by key residues such as Arg133 and Lys151, and requires phosphorylation (notably at Ser174) for its regulated release. eIF6 acts as an anti-association factor, sequestering mature 60S subunits to prevent their premature joining with 40S subunits, thereby maintaining translational fidelity and quality control; in the nucleolus, it facilitates 60S maturation through interactions with SBDS and EFL1, which, via GTP hydrolysis, evict eIF6 and license subunits for translation, thus dynamically coupling ribosome recycling to protein synthesis rates. eIF6 integrates mTOR/S6K signaling to align ribosome availability with cellular growth demands, and associates with RISC to promote miRNA-mediated translational silencing or recycling blocks, its deletion abolishes miRNA repression. eIF6 dysregulation is implicated in Shwachman-Diamond syndrome (where SBDS mutations trap eIF6 and hinder 60S-40S joining), while its overexpression in various cancers (such as colorectal and gastric) drives unrestrained translation and cyclin D1 upregulation, contributing to tumorigenesis and metabolic imbalance.

References
https://pubmed.ncbi.nlm.nih.gov/20356839/ https://pubmed.ncbi.nlm.nih.gov/35322020/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%