eEF1A Antibody [P21C20] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: MCF7, Lane 2: 293, Lane 3: PANC-1, Lane 4: C2C12

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50 kDa

Datasheet & SDS

Biological Description

Specificity
eEF1A Antibody [P21C20] detects endogenous levels of total eEF1A protein.

Clone
P21C20

Synonym(s)
Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor 1 A-1; eEF1A-1; Eukaryotic elongation factor 1 A-1; EEF1A1; EF1A; LENG7

Background
eEF1A serves as the GTPase subunit of the eukaryotic translation elongation factor 1 complex, delivering aminoacyl-tRNAs to the ribosomal A-site during protein synthesis across eukaryotes. It adopts a compact GTP-binding fold with three structural domains that undergo conformational shifts between GTP- and GDP-bound states to cycle between open and closed configurations. In its GTP-bound form, eEF1A forms a ternary complex with aminoacyl-tRNA and eEF1B guanine nucleotide exchange factor, promoting accurate codon-anticodon matching at the ribosome A-site followed by GTP hydrolysis triggered by the ribosome's sarcin-ricin loop and SRL GTPase-activating center. Post-hydrolysis, GDP-bound eEF1A dissociates, enabling eEF1B-mediated nucleotide exchange to reset the cycle while proofreading ensures fidelity through kinetic discrimination of cognate versus near-cognate tRNAs. Beyond elongation, eEF1A bundles actin filaments and stabilizes microtubules, interacting with F-actin, tubulin, and synaptic proteins to modulate cytoskeletal dynamics at growth cones and postsynaptic densities. Phosphorylation by Raf kinases at sites like Ser21 and Thr88 alters eEF1A stability, half-life, and apoptosis sensitivity in cancer cells, while heat shock activates eEF1A to recruit HSF1 to HSP70 promoters via non-coding HSR1 RNA, associate with RNA polymerase II during transcription elongation, stabilize HSP70 mRNA through 3'UTR binding, facilitate its nuclear export, and direct it to polysomes for stress granule-free translation. eEF1A isoforms exhibit tissue-specific expression with eEF1A1 ubiquitous and eEF1A2 enriched in brain and muscle, supporting proteostasis, signal transduction, and proteasomal delivery of misfolded proteins via interactions with HSC70 and Bag-6. Dysregulated eEF1A activity contributes to neurodegeneration and oncogenesis through altered translation fidelity and stress responses.

Background

eEF1A serves as the GTPase subunit of the eukaryotic translation elongation factor 1 complex, delivering aminoacyl-tRNAs to the ribosomal A-site during protein synthesis across eukaryotes. It adopts a compact GTP-binding fold with three structural domains that undergo conformational shifts between GTP- and GDP-bound states to cycle between open and closed configurations. In its GTP-bound form, eEF1A forms a ternary complex with aminoacyl-tRNA and eEF1B guanine nucleotide exchange factor, promoting accurate codon-anticodon matching at the ribosome A-site followed by GTP hydrolysis triggered by the ribosome's sarcin-ricin loop and SRL GTPase-activating center. Post-hydrolysis, GDP-bound eEF1A dissociates, enabling eEF1B-mediated nucleotide exchange to reset the cycle while proofreading ensures fidelity through kinetic discrimination of cognate versus near-cognate tRNAs. Beyond elongation, eEF1A bundles actin filaments and stabilizes microtubules, interacting with F-actin, tubulin, and synaptic proteins to modulate cytoskeletal dynamics at growth cones and postsynaptic densities. Phosphorylation by Raf kinases at sites like Ser21 and Thr88 alters eEF1A stability, half-life, and apoptosis sensitivity in cancer cells, while heat shock activates eEF1A to recruit HSF1 to HSP70 promoters via non-coding HSR1 RNA, associate with RNA polymerase II during transcription elongation, stabilize HSP70 mRNA through 3'UTR binding, facilitate its nuclear export, and direct it to polysomes for stress granule-free translation. eEF1A isoforms exhibit tissue-specific expression with eEF1A1 ubiquitous and eEF1A2 enriched in brain and muscle, supporting proteostasis, signal transduction, and proteasomal delivery of misfolded proteins via interactions with HSC70 and Bag-6. Dysregulated eEF1A activity contributes to neurodegeneration and oncogenesis through altered translation fidelity and stress responses.

References
https://pubmed.ncbi.nlm.nih.gov/25233275/ https://pubmed.ncbi.nlm.nih.gov/25233275/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%