E2F-1 Antibody [N6L18]

Catalog No.: F1062

    Home Primary Antibodies E2F-1 Antibody [N6L18]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:100-1:1000
    1:50-1:500
    1:50-1:500
    Application
    WB, IP, IHC, IF
    Reactivity
    Human, Mouse, Rat
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    60 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    E2F-1 Antibody [N6L18] detects endogenous levels of total E2F-1 protein.
    Clone
    N6L18
    Synonym(s)
    Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1; RBBP3
    Background
    E2F1, also known as E2F transcription factor 1, belongs to the E2F family of transcription factors that are central regulators of cell cycle progression and DNA synthesis. E2F1 contains a DNA-binding domain, a transcriptional activation domain, and a dimerization domain that allows binding to DP1/DP2 partners forming transcriptionally active heterodimers. The E2F1 protein specifically recognizes E2F-responsive elements in promoter regions of target genes essential for the G1/S transition. Through its interaction with the retinoblastoma (pRB) tumor suppressor, E2F1 activity is tightly controlled, as pRB binding represses E2F1-mediated transcription until phosphorylation releases E2F1 during the late G1 phase. E2F1 upregulates genes required for DNA replication, including those encoding cyclins, DNA polymerases, and dihydrofolate reductase. E2F1 exerts a dual role in cell fate by promoting apoptosis under stress conditions through both p53-dependent and independent mechanisms, involving transcriptional activation of pro-apoptotic genes such as APAF-1, p73, and caspases. E2F1 is phosphorylated and stabilized by ATM/ATR kinases in response to DNA damage, linking genotoxic stress signaling to apoptosis. Elevated E2F1 expression has been correlated with oncogenic transformation in multiple cancers, where its deregulation promotes uncontrolled proliferation and genomic instability. Moreover, aberrant E2F1 activity has been implicated in neuronal apoptosis, contributing to neurodegenerative disorders.
    References
    • https://pubmed.ncbi.nlm.nih.gov/22180494/
    • https://pubmed.ncbi.nlm.nih.gov/32787501/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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