DEC1 Antibody [D7J6] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Lane 1: HepG2

Usage Information

Dilution
WB1:100-1:1000 IP1:100-1:200

Application
WB, IP, ELISA

Reactivity
Human, Mouse, Rat

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
43 kDa

Datasheet & SDS

Biological Description

Specificity
DEC1 Antibody [D7J6] detects endogenous levels of total DEC1 protein.

Clone
D7J6

Synonym(s)
Class E basic helix-loop-helix protein 40; bHLHe40; Class B basic helix-loop-helix protein 2 (bHLHb2); DEC1; Enhancer-of-split and hairy-related protein 2 (SHARP-2); BHLHE40; BHLHB2; DEC1; SHARP2; STRA13

Background
DEC1 (BHLHE40) belongs to the basic helix-loop-helix family of transcription factors alongside DEC2, characterized by a DNA-binding basic domain and helix-loop-helix dimerization motif that recognizes E-box sequences (CACGTG) in target gene promoters to modulate circadian rhythm, differentiation, and stress responses. DEC1 organizes an N-terminal transactivation domain, central Orange domain for heterodimer selectivity, and nuclear localization signals flanking the bHLH region that confers specificity for class C E-boxes while repressing CLOCK-BMAL1 heterodimers essential for circadian oscillation. DEC1 transcriptionally represses pro-apoptotic genes under serum deprivation by stabilizing cyclin E and inhibiting caspase activation through selective suppression of mitochondrial pathways, while hypoxia-inducible factor signaling induces DEC1 expression that cooperates with HIF1α to transactivate VEGF and glycolytic enzymes for adaptive metabolic reprogramming. Interaction with p53 modulates DNA damage responses, where DEC1 attenuates MIC-1 induction but enhances survivin to balance survival versus death, alongside direct binding to TGF-β1 promoters that amplifies Smad3 phosphorylation and SNAIL expression driving epithelial-mesenchymal transition through N-cadherin upregulation and E-cadherin repression. DEC1 coordinates chondrocyte maturation and osteoblast differentiation during skeletal development, with rhythmic expression in the suprachiasmatic nucleus maintaining peripheral clock entrainment.

Background

DEC1 (BHLHE40) belongs to the basic helix-loop-helix family of transcription factors alongside DEC2, characterized by a DNA-binding basic domain and helix-loop-helix dimerization motif that recognizes E-box sequences (CACGTG) in target gene promoters to modulate circadian rhythm, differentiation, and stress responses. DEC1 organizes an N-terminal transactivation domain, central Orange domain for heterodimer selectivity, and nuclear localization signals flanking the bHLH region that confers specificity for class C E-boxes while repressing CLOCK-BMAL1 heterodimers essential for circadian oscillation. DEC1 transcriptionally represses pro-apoptotic genes under serum deprivation by stabilizing cyclin E and inhibiting caspase activation through selective suppression of mitochondrial pathways, while hypoxia-inducible factor signaling induces DEC1 expression that cooperates with HIF1α to transactivate VEGF and glycolytic enzymes for adaptive metabolic reprogramming. Interaction with p53 modulates DNA damage responses, where DEC1 attenuates MIC-1 induction but enhances survivin to balance survival versus death, alongside direct binding to TGF-β1 promoters that amplifies Smad3 phosphorylation and SNAIL expression driving epithelial-mesenchymal transition through N-cadherin upregulation and E-cadherin repression. DEC1 coordinates chondrocyte maturation and osteoblast differentiation during skeletal development, with rhythmic expression in the suprachiasmatic nucleus maintaining peripheral clock entrainment.

References
https://pubmed.ncbi.nlm.nih.gov/26819638/ https://pubmed.ncbi.nlm.nih.gov/12119049/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%