Cytochrome C Oxidase subunit VIc/COX6C Antibody [C2A20]

Application: Reactivity: Rat, Cow, Human

Usage Information

Dilution
WB1:1000 IF1:400 FCM1:2000

Application
WB, IF, FCM

Reactivity
Rat, Cow, Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
9 kDa

Datasheet & SDS

Biological Description

Specificity
Cytochrome C Oxidase subunit VIc/COX6C Antibody [C2A20] detects endogenous levels of total Cytochrome C Oxidase subunit VIc/COX6C protein.

Clone
C2A20

Synonym(s)
Cytochrome c oxidase subunit 6C, Cytochrome c oxidase polypeptide VIc, Cytochrome c oxidase subunit STA

Background
Cytochrome c oxidase subunit VIc (COX6C) is a small nuclear‑encoded component of cytochrome c oxidase (complex IV), the terminal enzyme of the mitochondrial respiratory chain that catalyzes electron transfer from reduced cytochrome c to molecular oxygen and contributes to the proton motive force driving oxidative phosphorylation. The protein is an integral inner mitochondrial membrane subunit that associates peripherally with the catalytic core formed by mitochondrially encoded COX1–3 and other nuclear subunits, and adopts a short transmembrane helix with matrix and intermembrane space extensions that help stabilize complex IV assembly and its interaction with neighboring respiratory complexes. COX6C participates in shaping the local environment of the catalytic center by affecting subunit packing and complex IV conformational dynamics, which influences the efficiency of electron transfer from cytochrome c to the binuclear heme a3–CuB center and the coupling of this redox chemistry to proton translocation across the inner membrane. Through its role in the intact holoenzyme, COX6C supports maintenance of mitochondrial membrane potential, ATP synthesis rates, and reactive oxygen species levels that are characteristic of high‑demand tissues, and its expression is enriched in heart, skeletal muscle, brain, and other metabolically active organs according to transcript and proteomic surveys. Altered COX6C expression has been reported in multiple disease states, including chronic kidney disease, diabetes, and several cancers, where up‑ or down‑regulation correlates with changes in oxidative phosphorylation markers and clinical parameters.

Background

Cytochrome c oxidase subunit VIc (COX6C) is a small nuclear‑encoded component of cytochrome c oxidase (complex IV), the terminal enzyme of the mitochondrial respiratory chain that catalyzes electron transfer from reduced cytochrome c to molecular oxygen and contributes to the proton motive force driving oxidative phosphorylation. The protein is an integral inner mitochondrial membrane subunit that associates peripherally with the catalytic core formed by mitochondrially encoded COX1–3 and other nuclear subunits, and adopts a short transmembrane helix with matrix and intermembrane space extensions that help stabilize complex IV assembly and its interaction with neighboring respiratory complexes. COX6C participates in shaping the local environment of the catalytic center by affecting subunit packing and complex IV conformational dynamics, which influences the efficiency of electron transfer from cytochrome c to the binuclear heme a3–CuB center and the coupling of this redox chemistry to proton translocation across the inner membrane. Through its role in the intact holoenzyme, COX6C supports maintenance of mitochondrial membrane potential, ATP synthesis rates, and reactive oxygen species levels that are characteristic of high‑demand tissues, and its expression is enriched in heart, skeletal muscle, brain, and other metabolically active organs according to transcript and proteomic surveys. Altered COX6C expression has been reported in multiple disease states, including chronic kidney disease, diabetes, and several cancers, where up‑ or down‑regulation correlates with changes in oxidative phosphorylation markers and clinical parameters.

References
https://pubmed.ncbi.nlm.nih.gov/35879322/ https://pubmed.ncbi.nlm.nih.gov/33527004/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%