CXXC5 Antibody [L11K10] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: MCF7, Lane 2: T47D, Lane 3: COS, Lane 4: H-4-II-E

Usage Information

Dilution
WB1:1000 IP1:100 IF1:1000

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
33 kDa

Datasheet & SDS

Biological Description

Specificity
CXXC5 Antibody [L11K10] detects endogenous levels of total CXXC5 protein.

Clone
L11K10

Synonym(s)
CXXC-type zinc finger protein 5; CXXC5

Background
CXXC5 belongs to the CXXC-type zinc finger protein family that recognizes unmethylated CpG dinucleotides and modulates Wnt/β-catenin signaling alongside BMP and TGF-β pathways during development and differentiation. It consists of a central CXXC domain flanked by acidic and proline-rich regions that mediate DNA binding and protein-protein interactions with Dishevelled, Smad3/4, and transcriptional co-regulators. CXXC5 suppresses canonical Wnt signaling by sequestering Dishevelled through direct binding that prevents LRP5/6 phosphorylation and β-catenin stabilization, thereby repressing target gene activation like Runx2 during osteoblast commitment while promoting non-canonical planar cell polarity through selective Dvl2 engagement. CXXC5 facilitates BMP-induced endothelial differentiation by coordinating VEGFR2 transcription via Smad1/5/8 nuclear translocation and enhances TGF-β-mediated apoptosis through Smad3 phosphorylation and Smad4 nuclear import that drives pro-apoptotic gene sets. CXXC5 transcriptionally activates MyoD and myogenin promoters to drive C2C12 myoblast fusion and sarcomere assembly, integrating with retinoic acid signaling for lineage specification. CXXC5 governs myelopoiesis through cytokine-driven maturation of hematopoietic precursors and supports angiogenesis via endothelial migration on VEGF gradients, with peak expression in embryonic mesoderm and adult bone marrow reflecting stage-specific roles in tissue morphogenesis.

Background

CXXC5 belongs to the CXXC-type zinc finger protein family that recognizes unmethylated CpG dinucleotides and modulates Wnt/β-catenin signaling alongside BMP and TGF-β pathways during development and differentiation. It consists of a central CXXC domain flanked by acidic and proline-rich regions that mediate DNA binding and protein-protein interactions with Dishevelled, Smad3/4, and transcriptional co-regulators. CXXC5 suppresses canonical Wnt signaling by sequestering Dishevelled through direct binding that prevents LRP5/6 phosphorylation and β-catenin stabilization, thereby repressing target gene activation like Runx2 during osteoblast commitment while promoting non-canonical planar cell polarity through selective Dvl2 engagement. CXXC5 facilitates BMP-induced endothelial differentiation by coordinating VEGFR2 transcription via Smad1/5/8 nuclear translocation and enhances TGF-β-mediated apoptosis through Smad3 phosphorylation and Smad4 nuclear import that drives pro-apoptotic gene sets. CXXC5 transcriptionally activates MyoD and myogenin promoters to drive C2C12 myoblast fusion and sarcomere assembly, integrating with retinoic acid signaling for lineage specification. CXXC5 governs myelopoiesis through cytokine-driven maturation of hematopoietic precursors and supports angiogenesis via endothelial migration on VEGF gradients, with peak expression in embryonic mesoderm and adult bone marrow reflecting stage-specific roles in tissue morphogenesis.

References
https://pubmed.ncbi.nlm.nih.gov/29928427/ https://pubmed.ncbi.nlm.nih.gov/25633194/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%