Claudin-1 Antibody [N2H22] | Primary Antibodies

Application: Reactivity: Human, Dog

Usage Information

Dilution
WB1:1000 IP1:200 IHC1:125 - 1:500 IF1:200

Application
WB, IP, IHC

Reactivity
Human, Dog

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
20 kDa

Positive Control	Human lung carcinoma; Human colon carcinoma; Human skin; A-431 cells
Negative Control	MCF7 cells

Datasheet & SDS

Biological Description

Specificity
Claudin-1 Antibody [N2H22] detects endogenous levels of total Claudin-1 protein.

Clone
N2H22

Synonym(s)
Claudin-1; Senescence-associated epithelial membrane protein; CLDN1; CLD1; SEMP1

Background
Claudin-1 (CLDN1), a key member of the 27-member claudin family of tight junction (TJ) tetraspan membrane proteins, is predominantly expressed in keratinocytes and epithelial tissues, where it polymerizes into paracellular strands to create a selective barrier that regulates ion and fluid permeability while maintaining apical-basal polarity. CLDN1 comprises four transmembrane helices (TM1–4), two extracellular loops (ECL1, containing conserved W30/W67/L75 for cis-strand interactions and electrostatic determinants for cation selectivity, and ECL2, featuring the Y147/V150 PDZ-binding motif), and short cytoplasmic N- and C-termini, with the C-terminal PDZ-binding tail anchoring TJs via interaction with scaffolding proteins ZO-1/2/3. Its function centers on homo- and heterophilic cis/trans interactions that generate TJ strands, sealing the paracellular space to restrict macromolecular passage while allowing selective ion flux (such as Na⁺ and Cl⁻), with C-terminal YV motifs engaging ZO-1 PDZ domains through hydrogen bonds and hydrophobic interactions to stabilize the complex and transduce signals influencing cell proliferation and migration. Claudin-1 preserves epidermal barrier integrity, supports epidermal differentiation, and maintains tissue homeostasis; its assembly into TJs is promoted by phosphorylation (e.g., by PKCθ) and disrupted by EphA2/ephrin signaling during epithelial-mesenchymal transition (EMT). CLDN1 overexpression enhances invasion and metastasis in colon and breast cancers (increasing MMP-2/9 activity, enabling anchorage-independence, and reducing E-cadherin), yet loss of CLDN1 predicts poor prognosis in skin squamous cell carcinoma.

Background

Claudin-1 (CLDN1), a key member of the 27-member claudin family of tight junction (TJ) tetraspan membrane proteins, is predominantly expressed in keratinocytes and epithelial tissues, where it polymerizes into paracellular strands to create a selective barrier that regulates ion and fluid permeability while maintaining apical-basal polarity. CLDN1 comprises four transmembrane helices (TM1–4), two extracellular loops (ECL1, containing conserved W30/W67/L75 for cis-strand interactions and electrostatic determinants for cation selectivity, and ECL2, featuring the Y147/V150 PDZ-binding motif), and short cytoplasmic N- and C-termini, with the C-terminal PDZ-binding tail anchoring TJs via interaction with scaffolding proteins ZO-1/2/3. Its function centers on homo- and heterophilic cis/trans interactions that generate TJ strands, sealing the paracellular space to restrict macromolecular passage while allowing selective ion flux (such as Na⁺ and Cl⁻), with C-terminal YV motifs engaging ZO-1 PDZ domains through hydrogen bonds and hydrophobic interactions to stabilize the complex and transduce signals influencing cell proliferation and migration. Claudin-1 preserves epidermal barrier integrity, supports epidermal differentiation, and maintains tissue homeostasis; its assembly into TJs is promoted by phosphorylation (e.g., by PKCθ) and disrupted by EphA2/ephrin signaling during epithelial-mesenchymal transition (EMT). CLDN1 overexpression enhances invasion and metastasis in colon and breast cancers (increasing MMP-2/9 activity, enabling anchorage-independence, and reducing E-cadherin), yet loss of CLDN1 predicts poor prognosis in skin squamous cell carcinoma.

References
https://pubmed.ncbi.nlm.nih.gov/24665401/ https://pubmed.ncbi.nlm.nih.gov/18036336/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%