CHOP Antibody [K6F19]

Catalog No.: F0132

    Home Primary Antibodies CHOP Antibody [K6F19]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:50
    Application
    WB, IP
    Reactivity
    Mouse
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    27 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    CHOP Antibody [K6F19] detects endogenous levels of total CHOP protein.
    Clone
    K6F19
    Synonym(s)
    DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; DDIT3; CHOP; CHOP10; GADD153
    Background
    CHOP (C/EBP homologous protein), also known as GADD153 or DDIT3, is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors central to cellular stress responses, particularly the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. CHOP is a 169-amino-acid protein featuring an N-terminal transcriptional activation domain and a C-terminal basic-leucine zipper (bZIP) domain that mediates DNA binding and dimerization. The bZIP domain allows heterodimerization with other C/EBPs and CREB/ATF family members, but proline and glycine substitutions in its basic region disrupt DNA binding at canonical C/EBP sites, enabling CHOP to function as a dominant-negative regulator of C/EBP-mediated transcription. CHOP is strongly upregulated under ER stress and promotes apoptosis by regulating target genes such as GADD34 and Ero1-Lα. GADD34 facilitates eIF2α dephosphorylation, restoring protein synthesis, while Ero1-Lα increases oxidative stress within the ER, together contributing to programmed cell death. Additionally, phosphorylation of CHOP at serine residues 79 and 82 by p38 MAP kinase enhances its transcriptional activity and is necessary for CHOP-induced apoptosis, particularly in macrophages.
    References
    • https://pubmed.ncbi.nlm.nih.gov/14685163/
    • https://pubmed.ncbi.nlm.nih.gov/29230213/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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