Home Primary Antibodies CHOP Antibody [C21N8]

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CHOP Antibody [C21N8]

Catalog No.: F2691

    Application: Reactivity:

    Usage Information

    Dilution
    1:2000
    Application
    WB, IP, IHC, IF
    Reactivity
    Human, Mouse
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Observed MW
    ~31 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    CHOP Antibody [C21N8] detects endogenous levels of total CHOP protein.
    Clone
    C21N8
    Synonym(s)
    C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; MGC4154; CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153
    Background
    CHOP (C/EBP Homologous Protein, also known as DDIT3 or GADD153) is a stress-inducible transcription factor of the C/EBP family that functions as a dominant-negative regulator of adipogenesis and a key mediator of ER stress-induced apoptosis. CHOP has an N-terminal transactivation/repression domain and a C-terminal bZIP domain, with proline/glycine insertions in the basic region that disrupt α-helical DNA binding and favor heterodimerization via its leucine zipper with C/EBPβ/δ or ATF/CREB family members, rather than homodimer formation. During ER stress, the PERK pathway phosphorylates eIF2α, reducing global translation but promoting ATF4 synthesis, which in turn upregulates CHOP expression. CHOP heterodimers bind C/EBP sites to repress cell cycle genes (causing G1/S arrest) and unique sites to activate pro-apoptotic targets such as GADD34 (restoring protein synthesis via eIF2α dephosphorylation), Ero1α (driving ER oxidative stress and ROS production), TRB3 (inhibiting Akt), and death receptors DR4/DR5 (triggering caspase-8 activation); CHOP can also tether AP-1 for indirect transcriptional activation. Loss of CHOP protects against ER stress-mediated apoptosis in conditions like neurodegeneration, diabetes, and ischemia, while its overexpression is implicated in cancer and fibrotic diseases.
    References
    • https://pubmed.ncbi.nlm.nih.gov/29230213/
    • https://pubmed.ncbi.nlm.nih.gov/14685163/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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