CHAF1A Antibody [G22G1] | Primary Antibodies

Application: Reactivity: Human

Lane 1: Hela, Lane 2: Hela (starvation, 24 h; nocodazole, 500ng/ml, 24 h), Lane 3: MCF7, Lane 4: K-562

Usage Information

Dilution
WB1:10000 IHC1:150 IF1:50 FCM1:20

Application
WB, IHC, IF, FCM

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
107 kDa

Datasheet & SDS

Biological Description

Specificity
CHAF1A Antibody [G22G1] detects endogenous levels of total CHAF1A protein.

Clone
G22G1

Synonym(s)
Chromatin assembly factor 1 subunit A; CAF-1 subunit p150; CHAF1A

Background
Chromatin assembly factor 1 subunit A (CHAF1A, also known as p150 CAF1) is a core component of the CAF‑1 complex, a histone chaperone system that couples nucleosome assembly to DNA replication and repair in the nucleus of eukaryotic cells. CHAF1A functions as a large modular scaffold that binds to proliferating cell nuclear antigen (PCNA) at the replication fork and associates with histones H3 and H4, enabling the deposition of newly synthesized (H3–H4)₂ tetramers onto nascent DNA and the rapid reassembly of chromatin during S‑phase progression. CHAF1A also interacts directly with the E3 ubiquitin ligase RAD18, promoting RAD18 recruitment to stalled forks and enhancing monoubiquitination of PCNA at K164, which licenses translesion DNA synthesis by Y‑family polymerases and supports fork restart and elongation under replication stress. CHAF1A also participates in interphase DNA repair contexts, where it helps restore chromatin organization after nucleotide excision repair and related pathways, thereby linking histone‑chaperone activity to genome‑integrity maintenance. CHAF1A antagonizes neuronal differentiation and sustains a proliferative, poorly differentiated state by reprogramming gene expression and upregulating polyamine metabolism, and elevated CHAF1A levels are associated with aggressive tumor phenotypes, drug resistance, and unfavorable outcomes in breast cancer and neuroblastoma.

Background

Chromatin assembly factor 1 subunit A (CHAF1A, also known as p150 CAF1) is a core component of the CAF‑1 complex, a histone chaperone system that couples nucleosome assembly to DNA replication and repair in the nucleus of eukaryotic cells. CHAF1A functions as a large modular scaffold that binds to proliferating cell nuclear antigen (PCNA) at the replication fork and associates with histones H3 and H4, enabling the deposition of newly synthesized (H3–H4)₂ tetramers onto nascent DNA and the rapid reassembly of chromatin during S‑phase progression. CHAF1A also interacts directly with the E3 ubiquitin ligase RAD18, promoting RAD18 recruitment to stalled forks and enhancing monoubiquitination of PCNA at K164, which licenses translesion DNA synthesis by Y‑family polymerases and supports fork restart and elongation under replication stress. CHAF1A also participates in interphase DNA repair contexts, where it helps restore chromatin organization after nucleotide excision repair and related pathways, thereby linking histone‑chaperone activity to genome‑integrity maintenance. CHAF1A antagonizes neuronal differentiation and sustains a proliferative, poorly differentiated state by reprogramming gene expression and upregulating polyamine metabolism, and elevated CHAF1A levels are associated with aggressive tumor phenotypes, drug resistance, and unfavorable outcomes in breast cancer and neuroblastoma.

References
https://pubmed.ncbi.nlm.nih.gov/34365742/ https://pubmed.ncbi.nlm.nih.gov/40025006/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%