CEA/CD66e Antibody [D17P1] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000-1:10000 IHC1:2000-1:3000 IF1:50 - 1:500

Application
WB, IHC, IF

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
76 kDa 160-200 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human gastric carcinoma tissue; Human colon tissue; Human colon carcinoma tissue; Human colon cancer; Stomach adenocarcinoma tissue; Thyroid gland carcinoma tissue; Glioma tissue; MCF7 cells; A549 cells; BxPC-3 cells
Negative Control	Hepatocellular carcinoma tissue; Normal tonsil tissue; Skeletal muscle tissue; Human colon tissue (boiled); PANC-1 cells; BxPC-3 cells (boiled); MCF7 cells (bioled)

Datasheet & SDS

Biological Description

Specificity
CEA/CD66e Antibody [D17P1] detects endogenous levels of total CEA/CD66e protein.

Clone
D17P1

Synonym(s)
CD66e; CEA; CEACAM5; Cell adhesion molecule CEACAM5; Carcinoembryonic antigen; Meconium antigen 100; CEA cell adhesion molecule 5

Background
CEA, also known as CEACAM5 or CD66e, is a GPI-anchored glycoprotein belonging to the carcinoembryonic antigen family within the immunoglobulin superfamily, alongside other CEACAM members. It features an extracellular N-terminal Ig-variable-like domain followed by six Ig-constant-like domains that are extensively glycosylated with sialylated N- and O-linked glycans. This structural arrangement enables calcium-independent homophilic and heterophilic adhesion through IgV-IgV interactions in both cis and trans configurations, with its elongated rod-like structure anchored to the plasma membrane via a C-terminal GPI anchor. CEA plays a pivotal role in cell-cell adhesion, influencing epithelial polarity, tissue architecture, and intercellular signaling. It also exhibits significant tumor-promoting activities, such as conferring resistance to detachment-induced cell death by activating focal adhesion kinase and Src, facilitating epithelial-mesenchymal transition by downregulating E-cadherin and upregulating N-cadherin and TWIST through the PI3K/Akt pathway, and enhancing cell migration, invasion, and metastasis through mechanisms that include liver colonization and immune evasion by impairing natural killer and T cell cytotoxicity. CEA is involved in several signaling cascades, notably those mediated by Src, FAK, PI3K/Akt, and EGFR, with its elevated expression closely associated with increased tumorigenicity in carcinomas such as those of the colon, breast, lung, and pancreas. CEA stands as a gold-standard biomarker for monitoring colorectal cancer recurrence and metastasis, where its overexpression also contributes to chemoresistance, promotes angiogenesis, and indicates poor prognosis.

Background

CEA, also known as CEACAM5 or CD66e, is a GPI-anchored glycoprotein belonging to the carcinoembryonic antigen family within the immunoglobulin superfamily, alongside other CEACAM members. It features an extracellular N-terminal Ig-variable-like domain followed by six Ig-constant-like domains that are extensively glycosylated with sialylated N- and O-linked glycans. This structural arrangement enables calcium-independent homophilic and heterophilic adhesion through IgV-IgV interactions in both cis and trans configurations, with its elongated rod-like structure anchored to the plasma membrane via a C-terminal GPI anchor. CEA plays a pivotal role in cell-cell adhesion, influencing epithelial polarity, tissue architecture, and intercellular signaling. It also exhibits significant tumor-promoting activities, such as conferring resistance to detachment-induced cell death by activating focal adhesion kinase and Src, facilitating epithelial-mesenchymal transition by downregulating E-cadherin and upregulating N-cadherin and TWIST through the PI3K/Akt pathway, and enhancing cell migration, invasion, and metastasis through mechanisms that include liver colonization and immune evasion by impairing natural killer and T cell cytotoxicity. CEA is involved in several signaling cascades, notably those mediated by Src, FAK, PI3K/Akt, and EGFR, with its elevated expression closely associated with increased tumorigenicity in carcinomas such as those of the colon, breast, lung, and pancreas. CEA stands as a gold-standard biomarker for monitoring colorectal cancer recurrence and metastasis, where its overexpression also contributes to chemoresistance, promotes angiogenesis, and indicates poor prognosis.

References
https://pubmed.ncbi.nlm.nih.gov/40364220/ https://pubmed.ncbi.nlm.nih.gov/24858421/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%