CD62L/L-Selectin Antibody [H14P17]

Application: Reactivity: Mouse

Usage Information

Dilution
WB1:1000 IHC1:150 - 1:600

Application
WB, IHC

Reactivity
Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
60-100 kDa

Positive Control	GL261 syngeneic tumor; Mouse thymus; LL/2 syngeneic tumor; CT26 syngeneic tumor; Mouse spleen; Mouse small intestine; Mouse lung; Mouse CD8+ T cells; EL4 cells; BA/F3 cells
Negative Control	Neuro-2a cells

Datasheet & SDS

Biological Description

Specificity
CD62L/L-Selectin Antibody [H14P17] detects endogenous levels of total CD62L/L-Selectin protein.

Clone
H14P17

Synonym(s)
L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1 (LAM-1); LECAM1; Lymphocyte antigen 22; lY-22; Lymphocyte surface MEL-14 antigen; CD62L; Sell; Lnhr; Ly-22; Ly22

Background
CD62L (L-Selectin, SELL) is a type-I transmembrane glycoprotein belonging to the selectin family of cell adhesion molecules (alongside E- and P-selectin), expressed on most circulating leukocytes including naive T cells, central memory T cells, monocytes, and neutrophils, where it mediates initial tethering and rolling on high endothelial venules (HEVs) in lymphoid tissues via weak calcium-dependent bonds with sulfated O-linked glycans on GlyCAM-1, CD34, and MAdCAM-1. It comprises an N-terminal C-type lectin domain for ligand carbohydrate recognition (key residues like Ca²⁺-coordinating Asp/Asp/Glu in epidermal growth factor-like domain), a single EGF-like domain, two consensus repeats for rigidity, a highly basic 17-residue cytoplasmic tail interacting with ERM proteins (ezrin-radixin-moesin), calmodulin, and PKC for cytoskeletal linkage and signaling, and a membrane-proximal extracellular stalk cleaved by ADAM17 protease during activation. Its primary functions involve facilitating lymphocyte homing to lymph nodes by enabling shear-resistant rolling on HEVs, promoting monocyte protrusion and transendothelial migration (TEM) through ERM-mediated actin remodeling, and signaling via Src kinases/FAK to enhance chemotaxis toward CCL21; post-activation shedding of CD62L is antigen-specific, downregulating HEV binding while enabling front-back polarity, cytotoxic granule mobilization (CD107a+), and perforin/granzyme release in effector T cells. CD62L distinguishes naive/central memory (CD62L^hi) from effector memory (CD62L^lo) T cells, regulates inflammation by balancing recruitment and emigration, and supports immune surveillance; shedding-resistant mutants impair lytic function by ~50%, linking ectodomain release to cytoskeleton reorganization. Dysregulated CD62L shedding contributes to chronic lymphocytic leukemia infiltration, excessive inflammation in atherosclerosis, and impaired antiviral immunity.

Background

CD62L (L-Selectin, SELL) is a type-I transmembrane glycoprotein belonging to the selectin family of cell adhesion molecules (alongside E- and P-selectin), expressed on most circulating leukocytes including naive T cells, central memory T cells, monocytes, and neutrophils, where it mediates initial tethering and rolling on high endothelial venules (HEVs) in lymphoid tissues via weak calcium-dependent bonds with sulfated O-linked glycans on GlyCAM-1, CD34, and MAdCAM-1. It comprises an N-terminal C-type lectin domain for ligand carbohydrate recognition (key residues like Ca²⁺-coordinating Asp/Asp/Glu in epidermal growth factor-like domain), a single EGF-like domain, two consensus repeats for rigidity, a highly basic 17-residue cytoplasmic tail interacting with ERM proteins (ezrin-radixin-moesin), calmodulin, and PKC for cytoskeletal linkage and signaling, and a membrane-proximal extracellular stalk cleaved by ADAM17 protease during activation. Its primary functions involve facilitating lymphocyte homing to lymph nodes by enabling shear-resistant rolling on HEVs, promoting monocyte protrusion and transendothelial migration (TEM) through ERM-mediated actin remodeling, and signaling via Src kinases/FAK to enhance chemotaxis toward CCL21; post-activation shedding of CD62L is antigen-specific, downregulating HEV binding while enabling front-back polarity, cytotoxic granule mobilization (CD107a+), and perforin/granzyme release in effector T cells. CD62L distinguishes naive/central memory (CD62L^hi) from effector memory (CD62L^lo) T cells, regulates inflammation by balancing recruitment and emigration, and supports immune surveillance; shedding-resistant mutants impair lytic function by ~50%, linking ectodomain release to cytoskeleton reorganization. Dysregulated CD62L shedding contributes to chronic lymphocytic leukemia infiltration, excessive inflammation in atherosclerosis, and impaired antiviral immunity.

References
https://pubmed.ncbi.nlm.nih.gov/31139190/ https://pubmed.ncbi.nlm.nih.gov/21829468/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%