Home Primary Antibodies CD11b + CD11c Antibody [C8C21]

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CD11b + CD11c Antibody [C8C21]

Catalog No.: F1536

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:2000
    Application
    IHC, IF
    Reactivity
    Rat
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years

    Datasheet & SDS

    Biological Description

    Specificity
    CD11b + CD11c Antibody [C8C21] detects endogenous levels of total CD11b and CD11c protein.
    Clone
    C8C21
    Synonym(s)
    Cdc2l1; Cdk11; Cdk11b; Cyclin-dependent kinase 11B; Cell division cycle 2-like protein kinase 1; Cyclin-dependent kinase 11; Galactosyltransferase-associated protein kinase p58/GTA; PITSLRE serine/threonine-protein kinase CDC2L1
    Background
    CD11b (αM, ITGAM) and CD11c (αX, ITGAX) are integrin α-subunits of the β2-integrin family (CD11/CD18), forming heterodimers CD11b/CD18 (Mac-1, CR3) and CD11c/CD18 (p150,95, CR4), respectively, primarily expressed on myeloid leukocytes, including monocytes, macrophages, neutrophils, and dendritic cells. Both feature a large extracellular region with a seven-bladed β-propeller domain, thigh domain, calf-1/calf-2 domains, and an inserted I-domain (von Willebrand factor A-like) as the primary ligand-binding site containing a metal ion-dependent adhesion site (MIDAS) with Mg²⁺ coordination by Asp, Ser, and Glu residues; CD11c/CD18 adopts bent (low-affinity), extended-closed, and extended-open (high-affinity) conformations regulated by talin binding to the β2-tail, with CD11c phosphorylation at α-chain Ser1158 enhancing activation. CD11b/CD18 binds iC3b, ICAM-1, fibrinogen to mediate leukocyte adhesion, transmigration, phagocytosis of opsonized particles, and respiratory burst via PI3K/Akt signaling, while CD11c/CD18 similarly facilitates iC3b/complement-mediated phagocytosis, DC antigen capture (e.g., CD47-deficient cells), and adhesion to endothelium/fibronectin, triggering cytoskeletal remodeling, NF-κB/MAPK activation, and cytokine production for innate/adaptive immunity. Dysregulation contributes to chronic inflammation (e.g., rheumatoid arthritis), hypertension via macrophage infiltration, and cancer progression through impaired immune surveillance.
    References
    • https://pubmed.ncbi.nlm.nih.gov/24129562/
    • https://pubmed.ncbi.nlm.nih.gov/27781085/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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