CBX8 Antibody [E24E8] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:200 CHIP1:50

Application
WB, IP, ChIP

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
65 kDa

Positive Control	NCCIT cells; A549 cells; NIH/3T3 cells; H-4-II-E cells; COS-7 cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
CBX8 Antibody [E24E8] detects endogenous levels of total CBX8 protein.

Clone
E24E8

Synonym(s)
Chromobox protein homolog 8; Polycomb 3 homolog (Pc3; hPc3); Rectachrome 1; CBX8; PC3; RC1

Background
CBX8, or Chromobox protein homolog 8, is a member of the CBX family within the Polycomb Repressive Complex 1 (PRC1), partnering with proteins like RING1 and BMI1 to maintain heritable gene silencing during development and stem cell self-renewal. As a canonical H3K27me3 reader, CBX8 recruits PRC1 to specific chromatin regions, ensuring stable transcriptional repression. CBX8 contains an N-terminal chromodomain that forms an aromatic cage for H3K27me3 binding, an AT-hook motif that interacts with the DNA minor groove, and a C-terminal region responsible for PRC1 assembly and localization to Polycomb group (PcG) bodies in the nucleus. Its primary functions revolve around dynamic chromatin repression: CBX8 binds to H3K27me3-marked loci, compacts chromatin through multivalent PRC1 interactions, and ubiquitinates histone H2AK119 to inhibit RNA polymerase II progression. During embryonic stem cell differentiation, CBX8 facilitates the transition from PRC1-CBX7 to PRC1-CBX8, thereby supporting the robust activation of lineage-specific genes, such as the Hox clusters. CBX8 modulates repression of the INK4A-ARF locus to enable cells to bypass oncogene-induced senescence, promotes proliferation by derepressing cyclin genes, and colocalizes with BMI1 and RING1B in nuclear PcG bodies. Disease relevance includes its role in MLL-AF9 leukemia, where CBX8 is essential for HOX gene maintenance, its involvement in fibroblast immortalization, and its influence on hematopoietic stem cell self-renewal, with overexpression driving senescence bypass and knockdown leading to G1 arrest.

Background

CBX8, or Chromobox protein homolog 8, is a member of the CBX family within the Polycomb Repressive Complex 1 (PRC1), partnering with proteins like RING1 and BMI1 to maintain heritable gene silencing during development and stem cell self-renewal. As a canonical H3K27me3 reader, CBX8 recruits PRC1 to specific chromatin regions, ensuring stable transcriptional repression. CBX8 contains an N-terminal chromodomain that forms an aromatic cage for H3K27me3 binding, an AT-hook motif that interacts with the DNA minor groove, and a C-terminal region responsible for PRC1 assembly and localization to Polycomb group (PcG) bodies in the nucleus. Its primary functions revolve around dynamic chromatin repression: CBX8 binds to H3K27me3-marked loci, compacts chromatin through multivalent PRC1 interactions, and ubiquitinates histone H2AK119 to inhibit RNA polymerase II progression. During embryonic stem cell differentiation, CBX8 facilitates the transition from PRC1-CBX7 to PRC1-CBX8, thereby supporting the robust activation of lineage-specific genes, such as the Hox clusters. CBX8 modulates repression of the INK4A-ARF locus to enable cells to bypass oncogene-induced senescence, promotes proliferation by derepressing cyclin genes, and colocalizes with BMI1 and RING1B in nuclear PcG bodies. Disease relevance includes its role in MLL-AF9 leukemia, where CBX8 is essential for HOX gene maintenance, its involvement in fibroblast immortalization, and its influence on hematopoietic stem cell self-renewal, with overexpression driving senescence bypass and knockdown leading to G1 arrest.

References
https://pubmed.ncbi.nlm.nih.gov/25500566/ https://pubmed.ncbi.nlm.nih.gov/17332741/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%