c-Kit Antibody [D16J9] | Primary Antibodies

Application: Reactivity: Human, Mouse

Lane 1: NCI-H526

Usage Information

Dilution
WB1:1000 IP1:50 IF1:400

Application
WB, IP, IF

Reactivity
Human, Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
120, 145 kDa

Datasheet & SDS

Biological Description

Specificity
c-Kit Antibody [D16J9] detects endogenous levels of total c-Kit protein.

Clone
D16J9

Synonym(s)
Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT

Background
c‑Kit is a type III receptor tyrosine kinase that belongs to the same subfamily as the PDGF, CSF‑1, and FLT3 receptors and is expressed in hematopoietic stem cells, mast cells, melanocytes, germ cells, interstitial cells of Cajal, and several neuronal populations. Upon binding of its ligand stem cell factor (SCF), c‑Kit undergoes dimerization and autophosphorylation on multiple cytoplasmic tyrosine residues, which serve as docking sites for SH2‑containing adaptor and effector proteins. Phosphorylated c‑Kit recruits and activates the p85 subunit of PI3‑kinase, PLCγ, SHP2, CrkL, and other signaling components, thereby initiating downstream PI3K/AKT, Ras/MAPK, JAK/STAT, and Src‑family kinase pathways that regulate cell survival, proliferation, migration, and differentiation. A specific tyrosine residue in the kinase insert region, Tyr719 (Y719), is essential for binding of the p85 subunit of PI3‑kinase and for c‑Kit‑associated PI3‑kinase activity, positioning this site as a key node for lipid‑mediated signaling. Activating mutations in the c‑Kit gene, particularly in exons encoding the juxtamembrane and kinase domains, are detected in a high proportion of gastrointestinal stromal tumors (GISTs) and in systemic mastocytosis, where constitutive receptor activity drives aberrant growth and survival of tumor cells. In contrast, loss‑of‑function or hypomorphic c‑Kit alleles are associated with developmental defects affecting hematopoiesis, melanogenesis, and gametogenesis, underscoring the receptor’s central role in tissue homeostasis.

Background

c‑Kit is a type III receptor tyrosine kinase that belongs to the same subfamily as the PDGF, CSF‑1, and FLT3 receptors and is expressed in hematopoietic stem cells, mast cells, melanocytes, germ cells, interstitial cells of Cajal, and several neuronal populations. Upon binding of its ligand stem cell factor (SCF), c‑Kit undergoes dimerization and autophosphorylation on multiple cytoplasmic tyrosine residues, which serve as docking sites for SH2‑containing adaptor and effector proteins. Phosphorylated c‑Kit recruits and activates the p85 subunit of PI3‑kinase, PLCγ, SHP2, CrkL, and other signaling components, thereby initiating downstream PI3K/AKT, Ras/MAPK, JAK/STAT, and Src‑family kinase pathways that regulate cell survival, proliferation, migration, and differentiation. A specific tyrosine residue in the kinase insert region, Tyr719 (Y719), is essential for binding of the p85 subunit of PI3‑kinase and for c‑Kit‑associated PI3‑kinase activity, positioning this site as a key node for lipid‑mediated signaling. Activating mutations in the c‑Kit gene, particularly in exons encoding the juxtamembrane and kinase domains, are detected in a high proportion of gastrointestinal stromal tumors (GISTs) and in systemic mastocytosis, where constitutive receptor activity drives aberrant growth and survival of tumor cells. In contrast, loss‑of‑function or hypomorphic c‑Kit alleles are associated with developmental defects affecting hematopoiesis, melanogenesis, and gametogenesis, underscoring the receptor’s central role in tissue homeostasis.

References
https://pubmed.ncbi.nlm.nih.gov/23678293/ https://pubmed.ncbi.nlm.nih.gov/23073628/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%