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C/EBPβ Antibody [B2A6]

Cat.No.: F3008

Application: Reactivity: Human, Monkey

Usage Information

Dilution
WB1:1000 IHC1:400 - 1:1600 IF1:400 - 1:1600 FCM1:3200 - 1:6400

Application
WB, IHC, IF, FCM

Reactivity
Human, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
46 (LAP), 48 (Full Length)

Datasheet & SDS

Biological Description

Specificity
C/EBPβ Antibody [B2A6] detects endogenous levels of total C/EBPβ protein.

Clone
B2A6

Synonym(s)
CEBPB; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein (LAP); Liver-enriched inhibitory protein (LIP); Nuclear factor NF-IL6; Transcription factor 5 (TCF-5); TCF5; PP9092

Background
C/EBPβ is a basic leucine-zipper (bZIP) transcription factor fundamental for the acute-phase response, adipogenesis, hematopoiesis, and immune regulation, functioning as homo- or heterodimers through its C-terminal leucine zipper that binds palindromic DNA consensus sequences via an N-terminal basic region, with three isoforms, LAP1, LAP2 (full-length activators), and LIP (truncated repressor), arising from alternative translation. It activates target genes such as IL-6, IL-8, and SAA by direct promoter binding and cooperative recruitment with NF-κB and STAT3 during inflammatory responses, promotes adipocyte differentiation by inducing PPARγ and C/EBPα, and regulates granulopoiesis through G-CSF and M-CSF expression. Phosphorylation at specific motifs, such as Thr235 by MAPK/p38, facilitates nuclear translocation and dimerization, while GSK3β-mediated Ser276 phosphorylation modulates its activation state in response to LPS and cytokines. C/EBPβ amplification can drive acute myeloid leukemia by disrupting PU.1 function, whereas deficiency impairs liver regeneration and macrophage polarization; overexpression of the LIP isoform is associated with poor prognosis in breast cancer due to its effects on cell cycle progression.

Background

C/EBPβ is a basic leucine-zipper (bZIP) transcription factor fundamental for the acute-phase response, adipogenesis, hematopoiesis, and immune regulation, functioning as homo- or heterodimers through its C-terminal leucine zipper that binds palindromic DNA consensus sequences via an N-terminal basic region, with three isoforms, LAP1, LAP2 (full-length activators), and LIP (truncated repressor), arising from alternative translation. It activates target genes such as IL-6, IL-8, and SAA by direct promoter binding and cooperative recruitment with NF-κB and STAT3 during inflammatory responses, promotes adipocyte differentiation by inducing PPARγ and C/EBPα, and regulates granulopoiesis through G-CSF and M-CSF expression. Phosphorylation at specific motifs, such as Thr235 by MAPK/p38, facilitates nuclear translocation and dimerization, while GSK3β-mediated Ser276 phosphorylation modulates its activation state in response to LPS and cytokines. C/EBPβ amplification can drive acute myeloid leukemia by disrupting PU.1 function, whereas deficiency impairs liver regeneration and macrophage polarization; overexpression of the LIP isoform is associated with poor prognosis in breast cancer due to its effects on cell cycle progression.

References
https://pubmed.ncbi.nlm.nih.gov/12006103/ https://pubmed.ncbi.nlm.nih.gov/19351437/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%