β-defensin 1 Antibody [K23F18] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
IHC1:1000

Application
WB, IHC, ELISA

Reactivity
Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	Human kidney
Negative Control

Datasheet & SDS

Biological Description

Specificity
β-defensin 1 Antibody [K23F18] detects endogenous levels of total β-defensin 1 protein.

Clone
K23F18

Synonym(s)
BD1, HBD1, DEFB1, Beta-defensin 1, BD-1, hBD-1

Background
β-defensin 1 (DEFB1) is a constitutively secreted, 4.8 kDa cationic antimicrobial peptide produced by epithelial cells lining the respiratory, urogenital, gastrointestinal, and skin mucosa. It adopts a compact β-hairpin structure stabilized by three invariant disulfide bonds (Cys5–Cys31, Cys10–Cys30, Cys19–Cys28), forming a rigid scaffold with an exposed cationic face, comprising residues Arg7, Lys20, and Lys27, that facilitates electrostatic attraction to anionic bacterial membranes, while a hydrophobic N-terminal tip (Phe2, Val3, Ile5) enables bilayer insertion. Reduced β-defensin 1^red with free Cys15/Cys43 thiols self-assembles into amyloid-like fibrils that create stable, bacteria-entrapping nets through cysteine crosslinking, immobilizing both Gram-positive and Gram-negative pathogens, including resistant strains, whereas oxidized β-defensin 1^ox selectively destabilizes Gram-negative LPS outer membranes. Both forms independently trigger platelet α-granule exocytosis, enhancing bacterial containment by 50–80% against S. aureus, and act as chemoattractants for neutrophils via CCR6, mimicking IL-8 to promote NET deployment, thus bridging immediate physical entrapment to adaptive immune activation without inducing resistance. Expressed as a monomer across barrier tissues, β-defensin 1 maintains microbiota homeostasis by exerting selective microbial pressure; its deficiency leads to Crohn’s disease dysbiosis through Paneth cell dysfunction, cystic fibrosis mucus overgrowth via CFTR interaction, and increased skin infections.

Background

β-defensin 1 (DEFB1) is a constitutively secreted, 4.8 kDa cationic antimicrobial peptide produced by epithelial cells lining the respiratory, urogenital, gastrointestinal, and skin mucosa. It adopts a compact β-hairpin structure stabilized by three invariant disulfide bonds (Cys5–Cys31, Cys10–Cys30, Cys19–Cys28), forming a rigid scaffold with an exposed cationic face, comprising residues Arg7, Lys20, and Lys27, that facilitates electrostatic attraction to anionic bacterial membranes, while a hydrophobic N-terminal tip (Phe2, Val3, Ile5) enables bilayer insertion. Reduced β-defensin 1^red with free Cys15/Cys43 thiols self-assembles into amyloid-like fibrils that create stable, bacteria-entrapping nets through cysteine crosslinking, immobilizing both Gram-positive and Gram-negative pathogens, including resistant strains, whereas oxidized β-defensin 1^ox selectively destabilizes Gram-negative LPS outer membranes. Both forms independently trigger platelet α-granule exocytosis, enhancing bacterial containment by 50–80% against S. aureus, and act as chemoattractants for neutrophils via CCR6, mimicking IL-8 to promote NET deployment, thus bridging immediate physical entrapment to adaptive immune activation without inducing resistance. Expressed as a monomer across barrier tissues, β-defensin 1 maintains microbiota homeostasis by exerting selective microbial pressure; its deficiency leads to Crohn’s disease dysbiosis through Paneth cell dysfunction, cystic fibrosis mucus overgrowth via CFTR interaction, and increased skin infections.

References
https://pubmed.ncbi.nlm.nih.gov/22441423/ https://pmc.ncbi.nlm.nih.gov/articles/PMC7149371/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%