Aquaporin 5 Antibody [N15B11] | Primary Antibodies

Application: Reactivity: Human

Lane 1: SW480 (RIPA lysis), Lane 2: SW480 (1%SDS Hot lysis)

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:1200

Application
WB, IHC

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
28 kDa 24 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Aquaporin 5 Antibody [N15B11] detects endogenous levels of total Aquaporin 5 protein.

Clone
N15B11

Synonym(s)
Aquaporin-5; AQP-5; AQP5

Background
Aquaporin 5 (AQP5) is a crucial member of the aquaporin family of water channel proteins, predominantly expressed in epithelial cells of secretory tissues such as salivary, lacrimal, and sweat glands, as well as in the lungs and eye. AQP5 functions as a homotetramer, with each of its four identical monomers comprising six transmembrane α-helices and two signature Asparagine-Proline-Alanine (NPA) motifs that are essential for selective water permeability while excluding protons and larger solutes. The selectivity filter, formed by the aromatic/arginine (ar/R) constriction region, ensures high water specificity and prevents ion leakage. AQP5 mediate rapid, osmotically driven water transport across epithelial membranes, which is vital for the secretion of fluids like saliva, tears, and airway surface liquid, thus maintaining tissue hydration and homeostasis. AQP5’s activity is tightly regulated by its trafficking to and from the plasma membrane, a process modulated by intracellular calcium and cAMP signaling, as well as interactions with regulatory proteins like ezrin, which are essential for its apical localization. Dysregulation of AQP5 is implicated in several diseases: in Sjögren’s syndrome, inflammatory mislocalization of AQP5 leads to impaired saliva and tear secretion, whereas in various cancers (including breast, lung, and colon), AQP5 overexpression enhances cell proliferation, migration, and metastasis through activation of pathways like Ras-MAPK.

Background

Aquaporin 5 (AQP5) is a crucial member of the aquaporin family of water channel proteins, predominantly expressed in epithelial cells of secretory tissues such as salivary, lacrimal, and sweat glands, as well as in the lungs and eye. AQP5 functions as a homotetramer, with each of its four identical monomers comprising six transmembrane α-helices and two signature Asparagine-Proline-Alanine (NPA) motifs that are essential for selective water permeability while excluding protons and larger solutes. The selectivity filter, formed by the aromatic/arginine (ar/R) constriction region, ensures high water specificity and prevents ion leakage. AQP5 mediate rapid, osmotically driven water transport across epithelial membranes, which is vital for the secretion of fluids like saliva, tears, and airway surface liquid, thus maintaining tissue hydration and homeostasis. AQP5’s activity is tightly regulated by its trafficking to and from the plasma membrane, a process modulated by intracellular calcium and cAMP signaling, as well as interactions with regulatory proteins like ezrin, which are essential for its apical localization. Dysregulation of AQP5 is implicated in several diseases: in Sjögren’s syndrome, inflammatory mislocalization of AQP5 leads to impaired saliva and tear secretion, whereas in various cancers (including breast, lung, and colon), AQP5 overexpression enhances cell proliferation, migration, and metastasis through activation of pathways like Ras-MAPK.

References
https://pubmed.ncbi.nlm.nih.gov/26837927/ https://pubmed.ncbi.nlm.nih.gov/18768791/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%