Home Primary Antibodies ApoA5 Antibody [H10J23]

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ApoA5 Antibody [H10J23]

Catalog No.: F9446

    Application: Reactivity:
    • F9446-wb
      Lane 1: HepG2, Lane 2: Human plasma

    Usage Information

    Dilution
    1:1000
    1:50
    Application
    WB, IP
    Reactivity
    Human
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    41 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    ApoA5 Antibody [H10J23] detects endogenous levels of total ApoA5 protein.
    Clone
    H10J23
    Synonym(s)
    Apolipoprotein A-V; Apo-AV; ApoA-V; Apolipoprotein A5; Regeneration-associated protein 3; APOA5; RAP3
    Background
    ApoA5 (apolipoprotein A-V) is a hydrophobic, amphipathic 343-amino-acid protein in the apolipoprotein gene family (APOA1/C3/A4 cluster) and serves as a crucial regulator of plasma triglyceride levels, particularly on triglyceride-rich lipoproteins such as chylomicrons and VLDL. ApoA5 features an N-terminal water-soluble helix bundle (residues 1–146), a C-terminal lipid-binding domain (295–343), and a proteoglycan-binding site (186–227) that facilitates interactions with lipoproteins and the endothelium, with coiled-coil domains enhancing lipid droplet and endothelial binding. ApoA5 binds triglyceride-rich lipoproteins and recruits lipoprotein lipase (LPL) via GPIHBP1 on capillary endothelial cells, stabilizing the lipolytic complex in membrane subdomains to accelerate chylomicron and VLDL lipolysis, and also suppresses ANGPTL3/8-mediated LPL inhibition through its C-terminal region. Within hepatocytes, ApoA5 targets ER-localized lipid droplets to regulate hepatic triglyceride flux, directing cytosolic lipid droplet formation over secretion and influencing lipoprotein assembly. Loss-of-function mutations or polymorphisms in APOA5 impair lipolysis and remnant clearance, leading to hypertriglyceridemia and increased cardiovascular risk.
    References
    • https://pubmed.ncbi.nlm.nih.gov/23307945/
    • https://pubmed.ncbi.nlm.nih.gov/26028042/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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