APC8 Antibody [E8M5] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000-1:50000 IP1:10-1:100 IF1:50-1:100

Application
WB, IP, IF

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
69 kDa

Datasheet & SDS

Biological Description

Specificity
APC8 Antibody [E8M5] detects endogenous levels of total APC8 protein.

Clone
E8M5

Synonym(s)
ANAPC8, CDC23, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, Cyclosome subunit 8, APC8

Background
CDC23 (ANAPC8) is a conserved tetratricopeptide repeat–containing subunit of the anaphase‑promoting complex/cyclosome (APC/C), a large multisubunit E3 ubiquitin ligase that governs mitotic progression and G1 maintenance by targeting key cell‑cycle regulators for ubiquitin‑dependent proteasomal degradation. The protein shares strong similarity with budding yeast Cdc23p and is required for progression through the G2/M transition, and its multiple TPR motifs form an elongated scaffold that mediates protein–protein interactions within the APC/C core and with regulatory coactivators, positioning CDC23 as a structural hub that helps organize substrate-recognition and catalytic modules. Within APC/C, CDC23 associates with other TPR subunits such as CDC27 and CDC16 and contributes to the assembly of the platform that recruits coactivators CDC20 and CDH1, which bind destruction-box or KEN-box motifs in substrates and bring them into proximity with the catalytic APC11–APC2 module that builds Lys11‑linked, and to a lesser extent Lys48‑ and Lys63‑linked, polyubiquitin chains on target proteins. These targets include securin and mitotic cyclins, whose timely ubiquitination and degradation at the metaphase–anaphase transition and during mitotic exit permit separase activation, sister chromatid separation, spindle disassembly, and reset of cyclin-dependent kinase activity, and CDC23 has been shown to bind mitotic cyclins directly as part of APC/C-mediated control of their turnover. The APC/C–CDC23 axis also participates in controlling the G1/S boundary and DNA replication competence by maintaining low CDK activity after mitosis and by coordinating with replication factors, as indicated by functional links between yeast Cdc23/Mcm10 and Cdc45 loading in replication initiation, underscoring how CDC23-related proteins connect ubiquitin-mediated proteolysis with replication licensing. CDC23 is expressed in proliferating cells and is essential for accurate chromosome segregation and genomic stability; altered expression or mutation perturbs APC/C function, leading to mitotic defects, aneuploidy, and aberrant proliferation, and elevated CDC23 levels correlate with aggressive phenotypes and poor outcomes in several cancers, including thyroid and other solid tumors, where CDC23 depletion suppresses proliferation, migration, and invasion and induces apoptotic markers.

Background

CDC23 (ANAPC8) is a conserved tetratricopeptide repeat–containing subunit of the anaphase‑promoting complex/cyclosome (APC/C), a large multisubunit E3 ubiquitin ligase that governs mitotic progression and G1 maintenance by targeting key cell‑cycle regulators for ubiquitin‑dependent proteasomal degradation. The protein shares strong similarity with budding yeast Cdc23p and is required for progression through the G2/M transition, and its multiple TPR motifs form an elongated scaffold that mediates protein–protein interactions within the APC/C core and with regulatory coactivators, positioning CDC23 as a structural hub that helps organize substrate-recognition and catalytic modules. Within APC/C, CDC23 associates with other TPR subunits such as CDC27 and CDC16 and contributes to the assembly of the platform that recruits coactivators CDC20 and CDH1, which bind destruction-box or KEN-box motifs in substrates and bring them into proximity with the catalytic APC11–APC2 module that builds Lys11‑linked, and to a lesser extent Lys48‑ and Lys63‑linked, polyubiquitin chains on target proteins. These targets include securin and mitotic cyclins, whose timely ubiquitination and degradation at the metaphase–anaphase transition and during mitotic exit permit separase activation, sister chromatid separation, spindle disassembly, and reset of cyclin-dependent kinase activity, and CDC23 has been shown to bind mitotic cyclins directly as part of APC/C-mediated control of their turnover. The APC/C–CDC23 axis also participates in controlling the G1/S boundary and DNA replication competence by maintaining low CDK activity after mitosis and by coordinating with replication factors, as indicated by functional links between yeast Cdc23/Mcm10 and Cdc45 loading in replication initiation, underscoring how CDC23-related proteins connect ubiquitin-mediated proteolysis with replication licensing. CDC23 is expressed in proliferating cells and is essential for accurate chromosome segregation and genomic stability; altered expression or mutation perturbs APC/C function, leading to mitotic defects, aneuploidy, and aberrant proliferation, and elevated CDC23 levels correlate with aggressive phenotypes and poor outcomes in several cancers, including thyroid and other solid tumors, where CDC23 depletion suppresses proliferation, migration, and invasion and induces apoptotic markers.

References
https://pubmed.ncbi.nlm.nih.gov/8423787/ https://pubmed.ncbi.nlm.nih.gov/21990323/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%