APC1 Antibody [C16G2] | Primary Antibodies

Application: Reactivity: Human, Monkey

Lane 1: 293T, Lane 2: MOLT-4, Lane 3: MCF7, Lane 4: COS7

Usage Information

Dilution
WB1:1000 IP1:200

Application
WB, IP

Reactivity
Human, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
216 kDa

Datasheet & SDS

Biological Description

Specificity
APC1 Antibody [C16G2] detects endogenous levels of total APC1 protein.

Clone
C16G2

Synonym(s)
Anaphase-promoting complex subunit 1; APC1; ANAPC1

Background
APC1 serves as the largest scaffold subunit within the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for mitotic progression and substrate-specific proteasomal degradation. Its architecture encompasses an N-terminal WD40 β-propeller domain linked via an α-helical solenoid to a central PC-repeat platform domain that anchors core APC/C components including APC4, APC5, and APC15, to form the structural platform. During prometaphase, CDK1 and PLK1 kinases target an N-terminal autoinhibitory loop in APC1 for multisite phosphorylation, inducing conformational release that exposes the coactivator-binding platform and permits CDC20 docking through direct interaction with APC1's WD40 repeats and APC8. This phospho-dependent switch allosterically rearranges the APC11-RING:APC2 catalytic module, enhancing recruitment of E2 enzymes UBE2C and UBE2S to initiate K11-linked polyubiquitination chains on D-box and KEN-box motifs of substrates like cyclin B, securin, and Aurora kinases. APC1 further stabilizes Cdh1 engagement in late mitosis and G1 via its extended platform interface, broadening substrate repertoire to include geminin and Cdt1 for licensing control. Mitotic phosphorylation peaks align with spindle assembly checkpoint satisfaction, with dephosphorylation by PP2A-B55δ enabling APC/C^Cdh1^ dominance for cytokinesis completion. The WD40 domain directly contacts CDC20's C-box, transmitting allosteric activation to the catalytic core while maintaining rotational flexibility for dynamic E2-substrate encounters. Ubiquitous expression supports universal applicability in cell cycle studies, with siRNA-mediated depletion yielding metaphase arrest and polyploidy.

Background

APC1 serves as the largest scaffold subunit within the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for mitotic progression and substrate-specific proteasomal degradation. Its architecture encompasses an N-terminal WD40 β-propeller domain linked via an α-helical solenoid to a central PC-repeat platform domain that anchors core APC/C components including APC4, APC5, and APC15, to form the structural platform. During prometaphase, CDK1 and PLK1 kinases target an N-terminal autoinhibitory loop in APC1 for multisite phosphorylation, inducing conformational release that exposes the coactivator-binding platform and permits CDC20 docking through direct interaction with APC1's WD40 repeats and APC8. This phospho-dependent switch allosterically rearranges the APC11-RING:APC2 catalytic module, enhancing recruitment of E2 enzymes UBE2C and UBE2S to initiate K11-linked polyubiquitination chains on D-box and KEN-box motifs of substrates like cyclin B, securin, and Aurora kinases. APC1 further stabilizes Cdh1 engagement in late mitosis and G1 via its extended platform interface, broadening substrate repertoire to include geminin and Cdt1 for licensing control. Mitotic phosphorylation peaks align with spindle assembly checkpoint satisfaction, with dephosphorylation by PP2A-B55δ enabling APC/C^Cdh1^ dominance for cytokinesis completion. The WD40 domain directly contacts CDC20's C-box, transmitting allosteric activation to the catalytic core while maintaining rotational flexibility for dynamic E2-substrate encounters. Ubiquitous expression supports universal applicability in cell cycle studies, with siRNA-mediated depletion yielding metaphase arrest and polyploidy.

References
https://pubmed.ncbi.nlm.nih.gov/27114510/ https://pubmed.ncbi.nlm.nih.gov/27601667/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%