Anti-Phospho-ULK1 (Ser555) Rabbit Antibody [N1G15]

Catalog No.: F0305

    Usage Information

    Dilution
    1:1000
    1:100
    Application
    WB, IP
    Reactivity
    Human, Mouse
    Source
    Rabbit
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃
    Storage (from the date of receipt)
    –20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    140-150 kDa
    Positive Control U-2 OS; MCF-7 (treated with CCCP, 100 μM, 2 h); C2C12 (treated with hydrogen peroxide, 10 mM, 5 min)
    Negative Control U-2 OS (ULK1 KO); MCF-7; C2C12

    Expression & Treatment Conditions

    Sample Treatment Conditions
    U-2OS AMPK Activator 991 (50 μM, 1 h)
    MCF-7 Oligomycin (0.5 μM, 30 min)
    MCF-7 Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (100 μM, 2 h)
    C2C12 Hydrogen peroxide (10 mM, 5 min)
    Click to view more sample data

    *For predicted expression levels of this protein in various human-derived cells and tissues, please refer to: http://www.proteinatlas.org