Home Primary Antibodies α-adaptin A Antibody [D21J17]

research use only

α-adaptin A Antibody [D21J17]

Catalog No.: F1926

    Application: Reactivity:

    Usage Information

    Dilution
    1:200
    1:1000-1:2000
    1:2000
    Application
    WB, IP, IHC, IF, FCM
    Reactivity
    Bovine, Hamster, Human, Mouse, Non-human primate, Rat
    Source
    Mouse Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    ~100 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    α-adaptin A Antibody [D21J17] detects endogenous levels of total α-adaptin A protein.
    Clone
    D21J17
    Synonym(s)
    100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; Adaptor protein complex AP-2 subunit alpha-1; ADTAA; AP2-ALPHA; AP2A1; CLAPA1
    Background
    α-Adaptin is a crucial subunit of the adaptor protein complex 2 (AP-2), which belongs to the family of adaptor protein complexes involved in intracellular vesicle transport. α-adaptin forms part of the heterotetrameric AP-2 complex, which consists of two large subunits (α and β2), a medium subunit (μ2), and a small subunit (σ2). The α-adaptin subunit contains a C-terminal appendage domain that functions as a scaffolding platform for endocytic accessory proteins. It also possesses regions that bind polyphosphoinositide-containing lipids, positioning AP-2 on the plasma membrane. AP-2 operates primarily in clathrin-dependent endocytosis by linking clathrin lattices to cargo proteins and membrane lipids and facilitating the formation of clathrin-coated vesicles (CCVs). The α and σ subunits contribute to recognizing specific endocytosis signal motifs, such as [ED]-X-X-X-L-[LI], which enables selective cargo capture. AP-2 also plays roles in recycling synaptic vesicle membranes, post-endocytic trafficking, and possibly ARF6-regulated non-clathrin pathways. Dysfunction or mutation in AP-2 components, such as α-adaptin, disrupts vesicle formation and endocytosis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/31353312/
    • https://pubmed.ncbi.nlm.nih.gov/8491205/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

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